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Cellosaurus 1205Lu (CVCL_5239)

[Text version]
Cell line name 1205Lu
Synonyms 1205-Lu; 1205 Lu; WM 1205LU; WM-1205LU; WM1205Lu; WM1205; LU1205; Lu1205; WC00058
Accession CVCL_5239
Resource Identification Initiative To cite this cell line use: 1205Lu (RRID:CVCL_5239)
Comments Problematic cell line: Partially contaminated. The stock formerly distributed by Coriell (WC00058) contains a significant amount of mouse DNA (DOI=10.4172/1948-5956.1000196). This is not the case of the original Wistar stock which was distributed by Rockland (DOI=10.4172/1948-5956.1000401).
Part of: Wistar Institute melanoma cell line collection.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00483.
Population: Caucasian.
Characteristics: Established from serial passaging of the parent cell line through immune deficient mice and harvesting lung metastasis.
Omics: Deep proteome analysis.
Omics: Exosome proteome analysis.
Omics: Transcriptome analysis by microarray.
Caution: The reported STR profile from Wistar of this cell line was changed at one point between February 2016 when we retrieved them and entered them in the Cellosaurus and May 2018. The major changes were: Amelogenin: X,Y->X, D16S539: 11,12->10,12, D7S820: 10,12->10, D8S1179: 11,12,14->12,14 and vWA: 16,17->17.
Derived from site: In situ; Skin; UBERON=UBERON_0002097.
Sequence variations
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (PubMed=16827748; PubMed=18632627; Wistar).
  • Mutation; HGNC; 1773; CDK4; Simple; p.Lys22Gln (c.64A>C); ClinVar=VCV000485487; Zygosity=Unspecified (Wistar).
HLA typing Source: PubMed=15592718
Class I
Class II
Disease Cutaneous melanoma (NCIt: C3510)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_8787 (WM793)
Sex of cell Male
Age at sampling 37Y
Category Cancer cell line
STR profile Source(s): ATCC; ESTDAB; Wistar

AmelogeninX (Wistar)
D7S82010 (Wistar)
10,12 (ATCC; ESTDAB)
D16S53910,12 (Wistar)
12 (ATCC)

Run an STR similarity search on this cell line
Web pages https://www.wistar.org/our-scientists/meenhard-herlyn

PubMed=2068080; DOI=10.1073/pnas.88.14.6028
Cornil I., Theodorescu D., Man S., Herlyn M., Jambrosic J.A., Kerbel R.S.
Fibroblast cell interactions with human melanoma cells affect tumor cell growth as a function of tumor progression.
Proc. Natl. Acad. Sci. U.S.A. 88:6028-6032(1991)

Juhasz I., Albelda S.M., Elder D.E., Murphy G.F., Adachi K., Herlyn D., Valyi-Nagy I.T., Herlyn M.
Growth and invasion of human melanomas in human skin grafted to immunodeficient mice.
Am. J. Pathol. 143:528-537(1993)

PubMed=15592718; DOI=10.1007/s00262-004-0561-5
Rodriguez T., Mendez R., Roberts C.H., Ruiz-Cabello F., Dodi I.A., Lopez Nevot M.A., Paco L., Maleno I., Marsh S.G.E., Pawelec G., Garrido F.
High frequency of homozygosity of the HLA region in melanoma cell lines reveals a pattern compatible with extensive loss of heterozygosity.
Cancer Immunol. Immunother. 54:141-148(2005)

PubMed=16827748; DOI=10.1111/j.1600-0749.2006.00322.x
Hoek K.S., Schlegel N.C., Brafford P., Sucker A., Ugurel S., Kumar R., Weber B.L., Nathanson K.L., Phillips D.J., Herlyn M., Schadendorf D., Dummer R.
Metastatic potential of melanomas defined by specific gene expression profiles with no BRAF signature.
Pigment Cell Res. 19:290-302(2006)

PubMed=18632627; DOI=10.1158/0008-5472.CAN-08-0235
Smalley K.S.M., Contractor R., Nguyen T.K., Xiao M., Edwards R., Muthusamy V., King A.J., Flaherty K.T., Bosenberg M.W., Herlyn M., Nathanson K.L.
Identification of a novel subgroup of melanomas with KIT/cyclin-dependent kinase-4 overexpression.
Cancer Res. 68:5743-5752(2008)

PubMed=18790768; DOI=10.1158/1535-7163.MCT-08-0431
Smalley K.S.M., Lioni M., Dalla Palma M., Xiao M., Desai B., Egyhazi Brage S., Hansson J., Wu H., King A.J., Van Belle P., Elder D.E., Flaherty K.T., Herlyn M., Nathanson K.L.
Increased cyclin D1 expression can mediate BRAF inhibitor resistance in BRAF V600E-mutated melanomas.
Mol. Cancer Ther. 7:2876-2883(2008)

Nair H.B., Bhaskaran S., Pathak S., Ghosh R., Betty D., Moore C.M., VandeBerg J.L.
1205Lu human metastatic melanoma cells, not human!
J. Cancer Sci. Ther. 5:119-119(2013)

PubMed=25950383; DOI=10.1111/pcmr.12380
Lazar I., Clement E., Ducoux-Petit M., Denat L., Soldan V., Dauvillier S., Balor S., Burlet-Schiltz O., Larue L., Muller C., Nieto L.
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines.
Pigment Cell Melanoma Res. 28:464-475(2015)

Brafford P., Sproesser K., Krepler C., Herlyn M.
1205Lu is human melanoma depending on the source.
J. Cancer Sci. Ther. 8:113-113(2016)

Cell line collections (Providers) ATCC; CRL-2812 - Discontinued
Coriell; WC00058 - Discontinued
Rockland; 51603 - Discontinued
Cell line databases/resources CLO; CLO_0001056
cancercelllines; CVCL_5239
Cell_Model_Passport; SIDM00972
Lonza; 900
Anatomy/cell type resources BTO; BTO:0005011
Biological sample resources BioSample; SAMN03471797
Encyclopedic resources Wikidata; Q54581163
Experimental variables resources EFO; EFO_0002810
Gene expression databases GEO; GSM109020
GEO; GSM109021
GEO; GSM188067
GEO; GSM188119
GEO; GSM188192
GEO; GSM188207
GEO; GSM188218
GEO; GSM188283
GEO; GSM188292
GEO; GSM952578
Polymorphism and mutation databases Cosmic; 686410
Cosmic; 905861
Cosmic; 972275
Cosmic; 1155274
Cosmic; 1155554
Cosmic; 1187583
Cosmic; 1303026
Cosmic; 1507616
Progenetix; CVCL_5239
Proteomic databases PRIDE; PXD004343
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number30