Cellosaurus TMNK-1 (CVCL_4W79)
| Cell line name | TMNK-1 | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Synonyms | TMNK1 | ||||||||||||||||||
| Accession | CVCL_4W79 | ||||||||||||||||||
| Resource Identification Initiative | To cite this cell line use: TMNK-1 (RRID:CVCL_4W79) | ||||||||||||||||||
| Comments | Group: Patented cell line. Registration: International Depositary Authority, Japanese National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology; FERM BP-8017. Characteristics: Because it has been transduced by two constructs containing LoxP sites, transfection with the Cre recombinase eliminates both the SV40 T gene and TERT thus reverting the cells to a non-immortal phenotype. Characteristics: Transduced with two retroviral vectors: 1) SSR#197 (MoMLV/LTR-LoxP-hTERT-IRES-EGFP-LoxP-MoMLV/LTR) expressing hTERT and eGFP flanked by two loxP sites and 2) SSR#69 (MoMLV/LTR-LoxP-HygroR-HSV/TK-IRES-SV40T-LoxP-Neo-MoMLV/LTR) expressing HygroR, HSV-1 TK and SV40 T-antigen flanked by two loxP sites. Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT. Genetic integration: Method=Transduction; Gene=UniProtKB; Q9QNF7; Human herpesvirus 1 TK. Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo. Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40). Derived from site: In situ; Liver; UBERON=UBERON_0002107. Cell type: Endothelial cell of hepatic sinusoid; CL=CL_1000398. | ||||||||||||||||||
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) | ||||||||||||||||||
| Sex of cell | Female | ||||||||||||||||||
| Age at sampling | Age unspecified | ||||||||||||||||||
| Category | Transformed cell line | ||||||||||||||||||
| STR profile | Source(s): JCRB=JCRB1564 Markers:
Run an STR similarity search on this cell line | ||||||||||||||||||
| Publications | PubMed=15167590; DOI=10.1097/01.TP.0000124286.82961.7E Patent=WO2008066199A1 | ||||||||||||||||||
| Cross-references | |||||||||||||||||||
| Cell line collections (Providers) | JCRB; JCRB1564 | ||||||||||||||||||
| Encyclopedic resources | Wikidata; Q54972700 | ||||||||||||||||||
| Entry history | |||||||||||||||||||
| Entry creation | 14-Dec-2015 | ||||||||||||||||||
| Last entry update | 19-Dec-2024 | ||||||||||||||||||
| Version number | 16 | ||||||||||||||||||