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Cellosaurus AC16 [Human hybrid cardiomyocyte] (CVCL_4U18)

[Text version]
Cell line name AC16 [Human hybrid cardiomyocyte]
Accession CVCL_4U18
Resource Identification Initiative To cite this cell line use: AC16 [Human hybrid cardiomyocyte] (RRID:CVCL_4U18)
Comments Group: Patented cell line.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); PTA-1500.
Characteristics: Hybrid of adult ventricular cardiomiocytes with a SV40 transformed, uridine auxotroph human fibroblast cell line devoid of mitochondrial DNA (PubMed=15913645).
Characteristics: Can differentiate when cultured in mitogen-free medium. The cells may be used to study developmental regulation of cardiomyocytes (Millipore=SCC109).
Doubling time: 24.49 hours (PubMed=15913645).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pRNS-1).
Derived from site: In situ; Heart, ventricle; UBERON=UBERON_0002082.
Cell type: Cardiomyocyte; CL=CL_0002495.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_4U14 (DWFb1-rho0)
Children:
CVCL_E2Q0 (HyCyte AC16 hRYR2_p.D179E_c.537T>G)CVCL_E1J3 (HyCyte AC16 KO-hCGAS)CVCL_D8DN (Ubigene AC16 RBM15 KO)
Category Hybrid cell line
STR profile Source(s): Millipore=SCC109

Markers:
AmelogeninX
CSF1PO9,11,12
D3S135817,18
D5S8189,11
D7S82010,11,12
D8S117912,14
D13S31712,13
D16S53911,13
D18S5112,17
D21S1132.2,33.2
FGA21,25
Penta D2.2,9
Penta E7,8,16
TH017,8,9.3
TPOX11
vWA16,18

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Publications

PubMed=15913645; DOI=10.1016/j.yjmcc.2005.03.003
Davidson M.M., Nesti C., Palenzuela L., Walker W.F., Hernandez E., Protas L., Hirano M., Isaac N.D.
Novel cell lines derived from adult human ventricular cardiomyocytes.
J. Mol. Cell. Cardiol. 39:133-147(2005)

Patent=US7223599
Davidson M.M.
Immortalization of human post-mitotic cells.
Patent number US7223599, 29-May-2007

Cross-references
Cell line collections (Providers) ATCC; CRL-3568
CLS; 305215
Millipore; SCC109
Encyclopedic resources Wikidata; Q54607923
Experimental variables resources EFO; EFO_0022630
Entry history
Entry creation22-Sep-2015
Last entry update10-Sep-2024
Version number10