Cellosaurus SK32 (CVCL_4708)
| Cell line name | SK32 |
|---|---|
| Accession | CVCL_4708 |
| Resource Identification Initiative | To cite this cell line use: SK32 (RRID:CVCL_4708) |
| Comments | Characteristics: Defective in Pex5 (PubMed=10771091). Characteristics: Selected using the 9-(1'-pyrene)nonanol/ultraviolet (P9OH/UV) method. P9OH acts as a photosensitizer. Normal cells which can incorporate the fluorescent fatty alcohol into lipids are killed by UV, but mutants who incorporate P9OH poorly are less susceptible to photosensitized killing. Characteristics: Transfected with a GFP C-terminally tagged with peroxisomal PTS1 signal (PubMed=10771091). Genetic integration: Method=Transfection; Gene=FPbase; 1XF1B; GFP (Note=avGFP; original A.victoria green fluorescent protein). Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Transformant: ChEBI; CHEBI:23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE). Derived from site: In situ; Ovary; UBERON=UBERON_0000992. Cell type: Epithelial cell of ovary; CL=CL_2000064. |
| Species of origin | Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029) |
| Hierarchy | Parent: CVCL_E3DU (CHOPS1a) |
| Sex of cell | Female |
| Age at sampling | Adult |
| Category | Spontaneously immortalized cell line |
| Publications | PubMed=10771091; DOI=10.1016/S0167-4889(00)00019-7 PubMed=11330054; DOI=10.1385/CBB:32:1-3:253 |
| Cross-references | |
| Anatomy/cell type resources | BTO; BTO:0003220 |
| Encyclopedic resources | Wikidata; Q54954659 |
| Entry history | |
| Entry creation | 04-Apr-2012 |
| Last entry update | 10-Sep-2024 |
| Version number | 11 |