ID   MARC-145
AC   CVCL_4540
SY   Marc-145; MARC 145; Marc 145; MARC145; Marc145; Meat Animal Research Center-145
DR   BTO; BTO:0006549
DR   CLO; CLO_0007536
DR   CLDB; cl5226
DR   CCRID; 5301MON-KCB08001YJ
DR   CLS; 305006
DR   GEO; GSM1263300
DR   GEO; GSM1263301
DR   GEO; GSM1263302
DR   GEO; GSM1263303
DR   KCB; KCB 200801YJ
DR   Wikidata; Q54904021
RX   Patent=US5690940;
RX   PubMed=8257302;
RX   PubMed=17081295;
RX   PubMed=23148668;
RX   PubMed=28046048;
RX   PubMed=29682543;
RX   PubMed=33268540;
RX   PubMed=34205087;
RX   PubMed=34737324;
CC   Group: Non-human primate cell line.
CC   Group: Patented cell line.
CC   Problematic cell line: Misidentified. Parent cell line (MA-104) has been shown to be from African Green monkey instead of Rhesus macaque.
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00621.
CC   Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-12219.
CC   Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-12230.
CC   Virology: Highly susceptible to infection by porcine reproductive and respiratory syndrome virus (PRRSV).
CC   Omics: Transcriptome analysis by microarray.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=60710; ! Chlorocebus pygerythrus (Vervet monkey)
HI   CVCL_3845 ! MA-104
SX   Sex unspecified
AG   Fetus
CA   Spontaneously immortalized cell line
DT   Created: 04-04-12; Last updated: 05-10-23; Version: 22
RX   Patent=US5690940;
RA   Joo H.S.;
RT   "Low pathogencity PRRS live virus vaccines and methods of preparation
RT   thereof.";
RL   Patent number US5690940, 25-Nov-1997.
RX   PubMed=8257302; DOI=10.1007/BF01313785;
RA   Kim H.S., Kwang J., Yoon I.J., Joo H.S., Frey M.L.;
RT   "Enhanced replication of porcine reproductive and respiratory syndrome
RT   (PRRS) virus in a homogeneous subpopulation of MA-104 cell line.";
RL   Arch. Virol. 133:477-483(1993).
RX   PubMed=17081295; DOI=10.1186/1743-422X-3-90;
RA   Cafruny W.A., Duman R.G., Wong G.H.W., Said S., Ward-Demo P.,
RA   Rowland R.R.R., Nelson E.A.;
RT   "Porcine reproductive and respiratory syndrome virus (PRRSV) infection
RT   spreads by cell-to-cell transfer in cultured MARC-145 cells, is
RT   dependent on an intact cytoskeleton, and is suppressed by
RT   drug-targeting of cell permissiveness to virus infection.";
RL   Virol. J. 3:90.1-90.17(2006).
RX   PubMed=23148668; DOI=10.1186/1743-422X-9-267;
RA   Provost C., Jia J.-J., Music N., Levesque C., Lebel M.-E.,
RA   del Castillo J.R.E., Jacques M., Gagnon C.A.;
RT   "Identification of a new cell line permissive to porcine reproductive
RT   and respiratory syndrome virus infection and replication which is
RT   phenotypically distinct from MARC-145 cell line.";
RL   Virol. J. 9:267.1-267.14(2012).
RX   PubMed=28046048; DOI=10.1371/journal.pone.0169391;
RA   Dotti S., Lombardo T., Villa R., Cacciamali A., Zanotti C.,
RA   Andreani N.A., Cinotti S., Ferrari M.;
RT   "Transformation and tumorigenicity testing of simian cell lines and
RT   evaluation of poliovirus replication.";
RL   PLoS ONE 12:E0169391-E0169391(2017).
RX   PubMed=29682543; DOI=10.1155/2018/4315861;
RA   Wu X.-J., Qi J., Cong X.-Y., Chen L., Hu Y., Yoo D.-W., Wang G.-S.,
RA   Tian F.-L., Li F., Sun W.-B., Chen Z., Guo L.-H., Wu J.-Q., Li J.,
RA   Wang J.-B., Zhao X.-M., Du Y.-J.;
RT   "Establishment and characterization of a high and stable porcine
RT   CD163-expressing MARC-145 cell line.";
RL   BioMed Res. Int. 2018:4315861.1-4315861.9(2018).
RX   PubMed=33268540; DOI=10.1128/jcm.01757-20;
RA   Yim-Im W., Huang H.-Y., Park J., Wang C., Calzada-Nova G., Gauger P.,
RA   Harmon K., Main R., Zhang J.-Q.;
RT   "Comparison of ZMAC and MARC-145 cell lines for improving porcine
RT   reproductive and respiratory syndrome virus isolation from clinical
RT   samples.";
RL   J. Clin. Microbiol. 59:e01757.20.1-e01757.20.15(2021).
RX   PubMed=34205087; DOI=10.3390/vaccines9060594;
RA   Xie J.-X., Vereecke N., Theuns S., Oh D.-Y., Vanderheijden N., Trus I.,
RA   Sauer J., Vyt P., Bonckaert C., Lalonde C., Provost C., Gagnon C.A.,
RA   Nauwynck H.J.;
RT   "Comparison of primary virus isolation in pulmonary alveolar
RT   macrophages and four different continuous cell lines for type 1 and
RT   type 2 porcine reproductive and respiratory syndrome virus.";
RL   Vaccines (Basel) 9:594.1-594.13(2021).
RX   PubMed=34737324; DOI=10.1038/s41598-021-00779-5;
RA   Lung O., Candlish R.C., Nebroski M., Kruckiewicz P., Buchanan C.,
RA   Moniwa M.;
RT   "High-throughput sequencing for species authentication and
RT   contamination detection of 63 cell lines.";
RL   Sci. Rep. 11:21657-21657(2021).