ID   LN-464
AC   CVCL_3962
SY   LN 464; LN464
DR   BTO; BTO_0004451
DR   BioSample; SAMN10988403
DR   cancercelllines; CVCL_3962
DR   Cell_Model_Passport; SIDM00058
DR   Cosmic; 849873
DR   Cosmic; 2367498
DR   Cosmic; 2516032
DR   DepMap; ACH-000676
DR   PharmacoDB; LN464_848_2019
DR   Wikidata; Q54902786
RX   PubMed=7693337;
RX   PubMed=8509230;
RX   PubMed=10416987;
RX   PubMed=22570425;
RX   PubMed=25984343;
RX   PubMed=31068700;
WW   Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
CC   Problematic cell line: Contaminated. The STR profile of ACN and LN-464 are identical. SNP analysis (DepMap) also shows identity of both cell lines. Personal communication of Hegi, Monika E. confirms the mismatch between the STR profile of this cell line and of the patient from which LN-464 was established. We believe this is a melanoma cell line based on the RNASeq expression profile of LN-464 (DepMap) which clusters with that of melanoma patient samples. The presence of the BRAF V600E while present at low frequency in gliomas is also a hallmark of melanomas. Originally thought to originate from the brain right parieto-occipital lobe of a 67 year old male patient with a glioblastoma.
CC   Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00624.
CC   Population: Caucasian.
CC   Doubling time: 35 hours (PubMed=25984343).
CC   Sequence variation: Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (DepMap=ACH-000676).
CC   Sequence variation: Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Asn184fs*6 (c.545_546insA); Zygosity=Heterozygous (PubMed=10416987; DepMap=ACH-000676).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Phe113Ser (c.338T>C); ClinVar=VCV000823709; Zygosity=Homozygous (PubMed=10416987; DepMap=ACH-000676).
CC   Omics: Genomics; Whole exome sequencing.
CC   Omics: Phenotyping; shRNA library screening.
ST   Source(s): PubMed=22570425
ST   Amelogenin: X
ST   CSF1PO: 11
ST   D13S317: 11,13
ST   D16S539: 11
ST   D18S51: 16
ST   D21S11: 28
ST   D3S1358: 16
ST   D5S818: 12
ST   D7S820: 10,13
ST   D8S1179: 12,13
ST   FGA: 22
ST   Penta D: 9
ST   Penta E: 10,14
ST   TH01: 9
ST   TPOX: 9,12
ST   vWA: 14,17,18
DI   NCIt; C3224; Melanoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
OI   CVCL_1068 ! ACN
SX   Sex unspecified
AG   Age unspecified
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 14-08-25; Version: 27
//
RX   PubMed=10416987; DOI=10.1111/j.1750-3639.1999.tb00536.x; PMCID=PMC8098486;
RA   Ishii, Nobuaki
RA   Maier, Daniel
RA   Merlo, Adrian
RA   Tada, Mitsuhiro
RA   Sawamura, Yutaka
RA   Diserens, Annie-Claire
RA   Van Meir, Erwin G.
RT   "Frequent co-alterations of TP53, p16/CDKN2A, p14ARF, PTEN tumor
RT   suppressor genes in human glioma cell lines.";
RL   Brain Pathol. 9:469-479(1999).
//
RX   PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103;
RA   Ghandi, Mahmoud
RA   Huang, Franklin W.
RA   Jane-Valbuena, Judit
RA   Kryukov, Gregory V.
RA   Lo, Christopher C.
RA   McDonald, E. Robert 3rd
RA   Barretina, Jordi Ginesta
RA   Gelfand, Ellen T.
RA   Bielski, Craig M.
RA   Li, Hao-Xin
RA   Hu, Kevin
RA   Andreev-Drakhlin, Alexander Y.
RA   Kim, Jaegil
RA   Hess, Julian M.
RA   Haas, Brian J.
RA   Aguet, Francois
RA   Weir, Barbara A.
RA   Rothberg, Michael V.
RA   Paolella, Brenton R.
RA   Lawrence, Michael Scott
RA   Akbani, Rehan
RA   Lu, Yi-Ling
RA   Tiv, Hong L.
RA   Gokhale, Prafulla C.
RA   de Weck, Antoine
RA   Mansour, Ali Amin
RA   Oh, Coyin
RA   Shih, Juliann
RA   Hadi, Kevin
RA   Rosen, Yanay
RA   Bistline, Jonathan
RA   Venkatesan, Kavitha
RA   Reddy, Anupama
RA   Sonkin, Dmitriy
RA   Liu, Manway
RA   Lehar, Joseph
RA   Korn, Joshua M.
RA   Porter, Dale A.
RA   Jones, Michael D.
RA   Golji, Javad
RA   Caponigro, Giordano
RA   Taylor, Jordan E.
RA   Dunning, Caitlin M.
RA   Creech, Amanda L.
RA   Warren, Allison C.
RA   McFarland, James M.
RA   Zamanighomi, Mahdi
RA   Kauffmann, Audrey
RA   Stransky, Nicolas
RA   Imielinski, Marcin
RA   Maruvka, Yosef E.
RA   Cherniack, Andrew D.
RA   Tsherniak, Aviad
RA   Vazquez, Francisca
RA   Jaffe, Jacob D.
RA   Lane, Andrew Alan
RA   Weinstock, David M.
RA   Johannessen, Cory M.
RA   Morrissey, Michael P.
RA   Stegmeier, Frank
RA   Schlegel, Robert
RA   Hahn, William Chun
RA   Getz, Gad
RA   Mills, Gordon B.
RA   Boehm, Jesse S.
RA   Golub, Todd Robert
RA   Garraway, Levi Alexander
RA   Sellers, William Raj
RT   "Next-generation characterization of the Cancer Cell Line
RT   Encyclopedia.";
RL   Nature 569:503-508(2019).
//
RX   PubMed=22570425; DOI=10.1093/neuonc/nos072; PMCID=PMC3367844;
RA   Bady, Pierre
RA   Diserens, Annie-Claire
RA   Castella, Vincent
RA   Kalt, Stefanie
RA   Heinimann, Karl
RA   Hamou, Marie-France
RA   Delorenzi, Mauro
RA   Hegi, Monika E.
RT   "DNA fingerprinting of glioma cell lines and considerations on
RT   similarity measurements.";
RL   Neuro-oncol. 14:701-711(2012).
//
RX   PubMed=7693337;
RA   Li, Hong
RA   Hamou, Marie-France
RA   de Tribolet, Nicolas
RA   Jaufeerally, Rebana
RA   Hofmann, Martin
RA   Diserens, Annie-Claire
RA   Van Meir, Erwin G.
RT   "Variant CD44 adhesion molecules are expressed in human brain
RT   metastases but not in glioblastomas.";
RL   Cancer Res. 53:5345-5349(1993).
//
RX   PubMed=25984343; DOI=10.1038/sdata.2014.35; PMCID=PMC4432652;
RA   Cowley, Glenn S.
RA   Weir, Barbara A.
RA   Vazquez, Francisca
RA   Tamayo, Pablo
RA   Scott, Justine A.
RA   Rusin, Scott
RA   East-Seletsky, Alexandra
RA   Ali, Levi D.
RA   Gerath, William F.J.
RA   Pantel, Sarah E.
RA   Lizotte, Patrick Hall
RA   Jiang, Guo-Zhi
RA   Hsiao, Jessica
RA   Tsherniak, Aviad
RA   Dwinell, Elizabeth
RA   Aoyama, Simon
RA   Okamoto, Michael
RA   Harrington, William
RA   Gelfand, Ellen T.
RA   Green, Thomas M.
RA   Tomko, Mark J.
RA   Gopal, Shuba
RA   Wong, Terence C.
RA   Li, Hu-Bo
RA   Howell, Sara
RA   Stransky, Nicolas
RA   Liefeld, Ted
RA   Jang, Dongkeun
RA   Bistline, Jonathan
RA   Meyers, Barbara Hill
RA   Armstrong, Scott Allen
RA   Anderson, Kenneth Carl
RA   Stegmaier, Kimberly
RA   Reich, Michael
RA   Pellman, David
RA   Boehm, Jesse S.
RA   Mesirov, Jill P.
RA   Golub, Todd Robert
RA   Root, David E.
RA   Hahn, William Chun
RT   "Parallel genome-scale loss of function screens in 216 cancer cell
RT   lines for the identification of context-specific genetic
RT   dependencies.";
RL   Sci. Data 1:140035.1-140035.12(2014).
//
RX   PubMed=8509230; DOI=10.1002/ijc.2910540329;
RA   Rimoldi, Donata
RA   Romero, Pedro
RA   Carrel, Stefan
RT   "The human melanoma antigen-encoding gene, MAGE-1, is expressed by
RT   other tumour cells of neuroectodermal origin such as glioblastomas and
RT   neuroblastomas.";
RL   Int. J. Cancer 54:527-528(1993).
//