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Cellosaurus LN-443 (CVCL_3960)

[Text version]
Cell line name LN-443
Synonyms LN 443; LN443
Accession CVCL_3960
Resource Identification Initiative To cite this cell line use: LN-443 (RRID:CVCL_3960)
Comments Problematic cell line: Contaminated. Shown to be a LN-444 derivative (PubMed=22570425). Originally thought to originate from a 66 year old male patient with a glioblastoma at the right fronto-temporal lobe.
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00451.
Population: Caucasian.
Doubling time: 40 hours (PubMed=25984343).
Omics: Deep exome analysis.
Omics: shRNA library screening.
Derived from site: In situ; Brain, left parietal lobe; UBERON=UBERON_0002802.
Sequence variations
  • Mutation; HGNC; 11998; TP53; Simple; p.His214Arg (c.641A>G); ClinVar=VCV000376615; Zygosity=Homozygous (DepMap=ACH-000673).
Genome ancestry Source: PubMed=30894373

Origin% genome
African3.39
Native American0
East Asian, North4.23
East Asian, South0
South Asian4.37
European, North27.1
European, South60.91
Disease Glioblastoma (NCIt: C3058)
Glioblastoma (ORDO: Orphanet_360)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_3961 (LN-444)
Sex of cell Female
Age at sampling 48Y
Category Cancer cell line
STR profile Source(s): PubMed=22570425

Markers:
AmelogeninX
CSF1PO10,12
D3S135815,17
D5S8189,12
D7S82010
D8S117913,14
D13S3178
D16S53910,11
D18S5115,16
D21S1128,30
FGA21,23
Penta D11,12
Penta E7,13
TH017,9
TPOX8
vWA18,19

Run an STR similarity search on this cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

PubMed=7693337
Li H., Hamou M.-F., de Tribolet N., Jaufeerally R., Hofmann M., Diserens A.-C., Van Meir E.G.
Variant CD44 adhesion molecules are expressed in human brain metastases but not in glioblastomas.
Cancer Res. 53:5345-5349(1993)

PubMed=8509230; DOI=10.1002/ijc.2910540329
Rimoldi D., Romero P., Carrel S.
The human melanoma antigen-encoding gene, MAGE-1, is expressed by other tumour cells of neuroectodermal origin such as glioblastomas and neuroblastomas.
Int. J. Cancer 54:527-528(1993)

PubMed=10416987; DOI=10.1111/j.1750-3639.1999.tb00536.x; PMCID=PMC8098486
Ishii N., Maier D., Merlo A., Tada M., Sawamura Y., Diserens A.-C., Van Meir E.G.
Frequent co-alterations of TP53, p16/CDKN2A, p14ARF, PTEN tumor suppressor genes in human glioma cell lines.
Brain Pathol. 9:469-479(1999)

PubMed=20593219; DOI=10.1007/s11060-010-0283-9
Blough M.D., Beauchamp D.C., Westgate M.R., Kelly J.J.P., Cairncross J.G.
Effect of aberrant p53 function on temozolomide sensitivity of glioma cell lines and brain tumor initiating cells from glioblastoma.
J. Neurooncol. 102:1-7(2011)

PubMed=22570425; DOI=10.1093/neuonc/nos072; PMCID=PMC3367844
Bady P., Diserens A.-C., Castella V., Kalt S., Heinimann K., Hamou M.-F., Delorenzi M., Hegi M.E.
DNA fingerprinting of glioma cell lines and considerations on similarity measurements.
Neuro-oncol. 14:701-711(2012)

PubMed=25984343; DOI=10.1038/sdata.2014.35; PMCID=PMC4432652
Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G.-Z., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E.T., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H.-B., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Meyers B.H., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035-140035(2014)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. 3rd, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

Cross-references
Cell line databases/resources cancercelllines; CVCL_3960
Cell_Model_Passport; SIDM01371
DepMap; ACH-000673
Anatomy/cell type resources BTO; BTO:0004449
Biological sample resources BioSample; SAMN03151823
BioSample; SAMN10988388
CRISP screens repositories BioGRID_ORCS_Cell_line; 407
Chemistry resources PharmacoDB; LN443_847_2019
Encyclopedic resources Wikidata; Q54902784
Polymorphism and mutation databases Cosmic; 849871
Cosmic; 2367496
Cosmic; 2516031
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number28