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Cellosaurus SKW 6.4 (CVCL_3796)

[Text version]
Cell line name SKW 6.4
Synonyms SKW6.4; Skw6.4; SKW 6-4; SKW6-Cl4; SKW6-CL4; SKW6 clone 4; SKW6-4
Accession CVCL_3796
Resource Identification Initiative To cite this cell line use: SKW 6.4 (RRID:CVCL_3796)
Comments Problematic cell line: Contaminated. Shown to be a WIL2-A3 derivative (ICLAC). Based on the STR profile. The exact WIL2 derivative has been arbitrarily chosen to be WIL2-A3 because this cell line was used in the lab where SKW 6.4 was derived (PubMed=305004). Originally thought to originate from the Daudi cell line.
Part of: Tumor Immunology Bank (TIB) collection from Salk (transferred to ATCC in 1981).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00617.
Population: Caucasian.
Characteristics: Produces and secretes IgM.
Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV).
Derived from sampling site: Spleen.
Sequence variations
  • Mutation; HGNC; 11272; SPTA1; Simple; p.Ser1163Ala (c.3487T>G); ClinVar=VCV000258929; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Gly59_Thr125del (c.175_375del199); Zygosity=Heterozygous (PubMed=19220422).
Disease Hereditary spherocytosis (NCIt: C97074)
Hereditary spherocytosis (ORDO: Orphanet_822)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_A7TT (WIL2-A3)
Sex of cell Male
Age at sampling 5Y
Category Transformed cell line
STR profile Source(s): ATCC

Markers:
AmelogeninX,Y
CSF1PO11,12
D5S81812,13
D7S8209,11
D13S31711
D16S53911,12
TH018,9.3
TPOX8,11
vWA17,20

Run an STR similarity search on this cell line
Web pages http://www.cells-talk.com/index.php/page/copelibrary?key=SKW6-Cl4
https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx
Publications

PubMed=305004; DOI=10.1038/271756a0
Kishimoto T., Hirano T., Kuritani T., Yamamura Y., Ralph P., Good R.A.
Induction of IgG production in human B lymphoblastoid cell lines with normal human T cells.
Nature 271:756-758(1978)

PubMed=6339249; DOI=10.1002/eji.1830130108
Saiki O., Ralph P.
Clonal differences in response to T cell replacing factor (TRF) for IgM secretion and TRF receptors in a human B lymphoblast cell line.
Eur. J. Immunol. 13:31-34(1983)

PubMed=6605913; DOI=10.1016/0165-2478(83)90049-4
Ralph P., Saiki O., Maurer D.H., Welte K.
IgM and IgG secretion in human B-cell lines regulated by B-cell-inducing factors (BIF) and phorbol ester.
Immunol. Lett. 7:17-23(1983)

Patent=US4730036
Ralph P., Welte K.
Purifications of B-cell inducing factor (BIF) and B-cell growth factor (BGF).
Patent number US4730036, 08-Mar-1988

PubMed=8509142
Cheng P.P.-J., Sreedharan S.P., Kishiyama J.L., Goetzl E.J.
The SKW 6.4 line of human B lymphocytes specifically binds and responds to vasoactive intestinal peptide.
Immunology 79:64-68(1993)

PubMed=19220422; DOI=10.1111/j.1600-0609.2009.01211.x
Kamihira S., Terada C., Sasaki D., Yanagihara K., Tsukasaki K., Hasegawa H., Yamada Y.
Aberrant p53 protein expression and function in a panel of hematopoietic cell lines with different p53 mutations.
Eur. J. Haematol. 82:301-307(2009)

Cross-references
Cell line collections ATCC; TIB-215
Cell line databases/resources CLO; CLO_0009067
Lonza; 83
Anatomy/cell type resources BTO; BTO:0001936
Chemistry resources ChEMBL-Cells; CHEMBL3307453
ChEMBL-Targets; CHEMBL614928
PubChem_Cell_line; CVCL_3796
Encyclopedic resources Wikidata; Q54954813
Entry history
Entry creation04-Apr-2012
Last entry update21-Mar-2023
Version number17