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Cellosaurus NCI-H69AR (CVCL_3513)

[Text version]
Cell line name NCI-H69AR
Synonyms NCI-H69 AR; NCI-H69/AR; H69AR; H-69AR
Accession CVCL_3513
Resource Identification Initiative To cite this cell line use: NCI-H69AR (RRID:CVCL_3513)
Comments Group: Patented cell line.
Part of: MD Anderson Cell Lines Project.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-11351.
Population: Caucasian.
Doubling time: ~24 hours (ATCC=CRL-11351).
Selected for resistance to: ChEBI; CHEBI_28748; Doxorubicin (Adriablastin; Adriamycin).
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Gly106_Arg108del (c.317_325delGGCAACCGT); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:9884; RB1; Simple; p.Glu748Ter (c.2242G>T); ClinVar=VCV000013078; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Glu171Ter (c.511G>T); ClinVar=VCV000634771; Zygosity=Homozygous (from parent cell line).
Disease Lung small cell carcinoma (NCIt: C4917)
Small cell lung cancer (ORDO: Orphanet_70573)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1579 (NCI-H69)
Children:
CVCL_4T78 (H69PR)
Sex of cell Male
Age at sampling 55Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-11351

Markers:
AmelogeninX
CSF1PO10,12
D2S133817
D3S135816
D5S81811,13
D7S8209
D8S117913
D13S31712
D16S53911
D18S5112,15
D19S43315,15.2
D21S1130,31.2
FGA24
Penta D9,11
Penta E12
TH018,9
TPOX10
vWA16,17

Run an STR similarity search on this cell line
Web pages https://tcpaportal.org/mclp/
Publications

PubMed=2436751
Mirski S.E.L., Gerlach J.H., Cole S.P.C.
Multidrug resistance in a human small cell lung cancer cell line selected in adriamycin.
Cancer Res. 47:2594-2598(1987)

PubMed=1314068; DOI=10.1038/bjc.1992.103; PMCID=PMC1977565
Cole S.P.C., Pinkoski M.J., Bhardwaj G., Deeley R.G.
Elevated expression of annexin II (lipocortin II, p36) in a multidrug resistant small cell lung cancer cell line.
Br. J. Cancer 65:498-502(1992)

Patent=US5489519
Deeley R.G., Cole S.P.C.
Multidrug resistance protein.
Patent number US5489519, 06-Feb-1996

PubMed=17426248; DOI=10.1158/1541-7786.MCR-06-0367
Olejniczak E.T., Van Sant C., Anderson M.G., Wang G., Tahir S.K., Sauter G., Lesniewski R., Semizarov D.
Integrative genomic analysis of small-cell lung carcinoma reveals correlates of sensitivity to bcl-2 antagonists and uncovers novel chromosomal gains.
Mol. Cancer Res. 5:331-339(2007)

PubMed=19446745; DOI=10.1016/j.cancergencyto.2009.01.016
Salido M., Arriola E., Carracedo A., Canadas I., Rovira A., Espinet B., Rojo F., Arumi-Uria M., Serrano S., Albanell J., Sole F.
Cytogenetic characterization of NCI-H69 and NCI-H69AR small cell lung cancer cell lines by spectral karyotyping.
Cancer Genet. Cytogenet. 191:97-101(2009)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076
Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

Cross-references
Cell line collections (Providers) ATCC; CRL-11351
Cell line databases/resources CLO; CLO_0003610
cancercelllines; CVCL_3513
IGRhCellID; H69AR
Anatomy/cell type resources BTO; BTO_0005367
Encyclopedic resources Wikidata; Q54872205
Gene expression databases ArrayExpress; E-MTAB-38
GEO; GSM169453
GEO; GSM170728
GEO; GSM827497
GEO; GSM986176
Polymorphism and mutation databases Progenetix; CVCL_3513
Entry history
Entry creation04-Apr-2012
Last entry update19-Dec-2024
Version number29