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Cellosaurus EJ-1 (CVCL_2893)

[Text version]
Cell line name EJ-1
Synonyms EJ1
Accession CVCL_2893
Resource Identification Initiative To cite this cell line use: EJ-1 (RRID:CVCL_2893)
Comments Problematic cell line: Contaminated. Shown to be a T24 derivative (DOI=10.11418/jtca1981.18.4_329; PubMed=20143388).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00194.
Population: Caucasian; Swedish.
Derived from site: In situ; Urinary bladder; UBERON=UBERON_0001255.
Sequence variations
  • Mutation; HGNC; 5173; HRAS; Simple; p.Gly12Val (c.35G>T); ClinVar=VCV000012600; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Tyr126Ter (c.378C>G); ClinVar=VCV000578988; Zygosity=Homozygous (from parent cell line).
Disease Bladder carcinoma (NCIt: C4912)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0554 (T24)
Sex of cell Female
Age at sampling 82Y
Category Cancer cell line
STR profile Source(s): JCRB=JCRB0710; PubMed=11416159; TKG=TKG 0441

Markers:
AmelogeninX
CSF1PO10,12
D5S81810,12
D7S82010,11
D8S117914
D13S31712
D16S5399
D18S5116,18
D21S1129
FGA17,22
TH016
TPOX8,11
vWA17

Run an STR similarity search on this cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

DOI=10.11418/jtca1981.18.4_329
Tanabe H., Takada Y., Minegishi D., Kurematsu M., Masui T., Mizusawa H.
Cell line individualization by STR multiplex system in the cell bank found cross-contamination between ECV304 and EJ-1/T24.
Tissue Cult. Res. Commun. 18:329-338(1999)

PubMed=11416159; DOI=10.1073/pnas.121616198; PMCID=PMC35459
Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

Cross-references
Cell line collections (Providers) JCRB; JCRB0710
RCB; RCB2137 - Discontinued
TKG; TKG 0441
Cell line databases/resources cancercelllines; CVCL_2893
Anatomy/cell type resources BTO; BTO:0003639
Biological sample resources BioSample; SAMN03151938
Encyclopedic resources Wikidata; Q54832168
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number24