ID   MT-4
AC   CVCL_2632
SY   MT4
DR   BTO; BTO:0003030
DR   CLO; CLO_0007891
DR   CLDB; cl3594
DR   CLDB; cl3599
DR   BioSample; SAMN03470803
DR   BioSample; SAMN03472442
DR   ChEMBL-Cells; CHEMBL3308375
DR   ChEMBL-Targets; CHEMBL388
DR   Cosmic; 1191697
DR   ECACC; 08081402
DR   HIVReagentProgram; ARP-120
DR   KCB; KCB 200308YJ
DR   PubChem_Cell_line; CVCL_2632
DR   Wikidata; Q54906940
RX   CelloPub=CLPUB00542;
RX   PubMed=1364232;
RX   PubMed=1765142;
RX   PubMed=2413616;
RX   PubMed=8927062;
RX   PubMed=8957066;
RX   PubMed=9261343;
RX   PubMed=29892436;
RX   PubMed=31554688;
RX   PubMed=32938764;
CC   Problematic cell line: Partially contaminated. Two different lots formerly distributed by NIH-ARP; 13 P7 3/9/92 and 150048; were shown not to be authentic MT-4 (PubMed=9261343; PubMed=31554688).
CC   Population: Japanese.
CC   Characteristics: Was established by co-cultivation of cells from a 50 year old male T-cell leukemia patient with cord leukocytes from a male infant (CelloPub=CLPUB00542).
CC   Virology: HTLV-1 producing cell line. Expresses Tax. Is permissive for replication of an HIV-1 gp41 mutant lacking the cytoplasmic tail.
CC   Doubling time: ~30 hours (Note=Lot 102799) (JCRB=JCRB1216).
CC   Transformant: NCBI_TaxID; 11908; Human T-lymphotropic virus 1 (HTLV-1).
CC   Discontinued: JCRB; JCRB0135; probable.
CC   Discontinued: JCRB; JCRB1216; probable.
CC   Discontinued: JCRB; NIHS0145; true.
CC   Discontinued: JCRB; NIHS0529; true.
CC   Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
CC   Cell type: T-cell; CL=CL_0000084.
ST   Source(s): JCRB; PubMed=31554688; PubMed=32938764
ST   Amelogenin: X,Y
ST   CSF1PO: 11,12
ST   D13S317: 12
ST   D16S539: 9,12
ST   D18S51: 13
ST   D21S11: 28,30.3
ST   D3S1358: 17
ST   D5S818: 10,11
ST   D7S820: 8,9.3,10 (JCRB_JCRB1216)
ST   D7S820: 8,10 (JCRB_JCRB0135; PubMed=31554688; PubMed=32938764)
ST   D8S1179: 10,15
ST   FGA: 23
ST   Penta D: 10,13
ST   Penta E: 5,15
ST   TH01: 7
ST   TPOX: 11
ST   vWA: 17,18
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Male
AG   <1D
CA   Transformed cell line
DT   Created: 04-04-12; Last updated: 29-06-23; Version: 23
RX   CelloPub=CLPUB00542;
RA   Miyoshi I., Taguchi H., Kubonishi I., Yoshimoto S., Ohtsuki Y.,
RA   Shiraishi Y., Akagi T.;
RT   "Type C virus-producing cell lines derived from adult T cell leukemia.";
RL   Gann Monogr. Cancer Res. 28:219-228(1982).
RX   PubMed=1364232;
RA   Gyuris A., Vajda G., Foldes I.;
RT   "Establishment of an MT4 cell line persistently producing infective
RT   HIV-1 particles.";
RL   Acta Microbiol. Hung. 39:271-279(1992).
RX   PubMed=1765142; DOI=10.1016/0014-5793(91)81402-t;
RA   Orita S., Saiga A., Takagi S., Tanaka T., Okumura K., Aono Y.,
RA   Hinuma Y., Igarashi H.;
RT   "A novel alternatively spliced viral mRNA transcribed in cells
RT   infected with human T cell leukemia virus type 1 is mainly responsible
RT   for expressing p21X protein.";
RL   FEBS Lett. 295:127-134(1991).
RX   PubMed=2413616; DOI=10.1016/0042-6822(85)90010-8;
RA   Harada S., Koyanagi Y., Yamamoto N.;
RT   "Infection of human T-lymphotropic virus type-I (HTLV-I)-bearing MT-4
RT   cells with HTLV-III (AIDS virus): chronological studies of early
RT   events.";
RL   Virology 146:272-281(1985).
RX   PubMed=8927062;
RA   Susloparov M.A., Krendel'shchikov A.V., Bakhtina M.M., Bedristov A.I.,
RA   Babkin I.V., Gutorov V.V., Eroshkin A.M., Mamaeva O.A.;
RT   "Cloning of proviral DNA sequences of the human T-cell leukemia virus
RT   type 1 (HTLV-1) from the genome of cultured MT-4 cells.";
RL   Mol. Genet. Mikrobiol. Virusol. 1996:32-39(1996).
RX   PubMed=8957066; DOI=10.1111/j.1349-7006.1996.tb03112.x;
RA   Kawasaki N., Matsuo Y., Yoshino T., Yanai H., Oka T., Teramoto N.,
RA   Liu C., Kondo E., Minowada J., Akagi T.;
RT   "Metastatic potential of lymphoma/leukemia cell lines in SCID mice is
RT   closely related to expression of CD44.";
RL   Jpn. J. Cancer Res. 87:1070-1077(1996).
RX   PubMed=9261343; DOI=10.1128/JVI.71.9.6277-6278.1997;
RA   Jeang K.-T., Derse D.D., Matocha M., Sharma O.;
RT   "Expression status of Tax protein in human T-cell leukemia virus type
RT   1-transformed MT4 cells: recall of MT4 cells distributed by the NIH
RT   AIDS Research and Reference Reagent Program.";
RL   J. Virol. 71:6277-6278(1997).
RX   PubMed=29892436; DOI=10.1098/rsos.172472;
RA   Shioda S., Kasai F., Watanabe K., Kawakami K., Ohtani A., Iemura M.,
RA   Ozawa M., Arakawa A., Hirayama N., Kawaguchi E., Tano T., Miyata S.,
RA   Satoh M., Shimizu N., Kohara A.;
RT   "Screening for 15 pathogenic viruses in human cell lines registered at
RT   the JCRB Cell Bank: characterization of in vitro human cells by viral
RT   infection.";
RL   R. Soc. Open Sci. 5:172472-172472(2018).
RX   PubMed=31554688; DOI=10.1128/JVI.01390-19;
RA   Fernandez M.V., Delviks-Frankenberry K.A., Scheiblin D.A., Happel C.,
RA   Pathak V.K., Freed E.O.;
RT   "Authentication analysis of MT-4 cells distributed by the NIH AIDS
RT   Reagent Program.";
RL   J. Virol. 93:e01390.19.1-e01390.19.8(2019).
RX   PubMed=32938764; DOI=10.1128/JVI.01334-20;
RA   Fernandez M.V., Hoffman H.K., Pezeshkian N., Tedbury P.R.,
RA   van Engelenburg S.B., Freed E.O.;
RT   "Elucidating the basis for permissivity of the MT-4 T-cell line to
RT   replication of an HIV-1 mutant lacking the gp41 cytoplasmic tail.";
RL   J. Virol. 94:e01334.20.1-e01334.20.27(2020).