ID   HCT 8
AC   CVCL_2478
SY   HCT-8; HCT8
DR   BTO; BTO_0002255
DR   CLO; CLO_0003667
DR   EFO; EFO_0002189
DR   CLDB; cl1577
DR   CLDB; cl1578
DR   CLDB; cl4949
DR   CLDB; cl5197
DR   AddexBio; C0009017/4907
DR   ArrayExpress; E-MTAB-38
DR   ArrayExpress; E-MTAB-2706
DR   BCRC; 60303
DR   BioSample; SAMN03473138
DR   cancercelllines; CVCL_2478
DR   CCRID; 1101HUM-PUMC000104
DR   CCRID; 3101HUMTCHu18
DR   CCRID; 4201HUM-CCTCC00201
DR   CCTCC; GDC0201
DR   Cell_Model_Passport; SIDM00786
DR   ChEMBL-Cells; CHEMBL3308035
DR   ChEMBL-Targets; CHEMBL613510
DR   CLS; 300210
DR   ColonAtlas; HCT8
DR   Cosmic; 887226
DR   Cosmic; 948853
DR   Cosmic; 983738
DR   Cosmic; 988873
DR   Cosmic; 1043814
DR   Cosmic; 1310946
DR   Cosmic; 1479593
DR   Cosmic; 1609484
DR   Cosmic; 1676735
DR   Cosmic; 2267316
DR   Cosmic; 2301980
DR   Cosmic; 2651867
DR   DepMap; ACH-001084
DR   ECACC; 90032006
DR   EGA; EGAS00001000610
DR   GEO; GSM206504
DR   GEO; GSM274709
DR   GEO; GSM274710
DR   GEO; GSM274723
DR   GEO; GSM513910
DR   GEO; GSM514294
DR   GEO; GSM827344
DR   GEO; GSM1346876
DR   GEO; GSM1374523
DR   GEO; GSM1374524
DR   GEO; GSM1448162
DR   ICLC; HTL99005
DR   IGRhCellID; HCT8
DR   IZSLER; BS TCL 97
DR   KCB; KCB 2011103YJ
DR   KCLB; 10244
DR   LINCS_LDP; LCL-1198
DR   MetaboLights; MTBLS227
DR   PharmacoDB; HCT8_519_2019
DR   Progenetix; CVCL_2478
DR   PubChem_Cell_line; CVCL_2478
DR   Wikidata; Q54882008
RX   PubMed=77569;
RX   PubMed=8422623;
RX   PubMed=9809040;
RX   PubMed=10674020;
RX   PubMed=20215515;
RX   PubMed=20570890;
RX   PubMed=24755471;
RX   PubMed=25485619;
RX   PubMed=25877200;
RX   PubMed=25926053;
RX   PubMed=25944804;
RX   PubMed=26537799;
RX   PubMed=26589293;
RX   PubMed=28196595;
RX   PubMed=31068700;
RX   PubMed=33389257;
WW   Info; MCLP; -; https://tcpaportal.org/mclp/
CC   Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
CC   Part of: MD Anderson Cell Lines Project.
CC   Population: Caucasian.
CC   Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
CC   Doubling time: 27.49 hours (PubMed=25944804).
CC   HLA typing: A*03,09; B*08 (PubMed=77569).
CC   HLA typing: A*02:01,24:02; B*08:01,35:01; C*04:01,07:01 (PubMed=26589293).
CC   Microsatellite instability: Instable (MSI) (PubMed=10674020; PubMed=24755471; PubMed=25926053).
CC   Sequence variation: Mutation; HGNC; HGNC:583; APC; Simple; p.Arg727Met (c.2180G>T); Zygosity=Unspecified (PubMed=24755471).
CC   Sequence variation: Mutation; HGNC; HGNC:583; APC; Simple; p.Lys993Asn (c.2979G>T); Zygosity=Unspecified (PubMed=24755471).
CC   Sequence variation: Mutation; HGNC; HGNC:583; APC; Simple; p.Ile1417Leufs*2 (c.4248delC); Zygosity=Unspecified (PubMed=24755471).
CC   Sequence variation: Mutation; HGNC; HGNC:583; APC; Simple; p.Arg2166Ter (c.6496C>T); ClinVar=VCV000438883; Zygosity=Unspecified (PubMed=24755471).
CC   Sequence variation: Mutation; HGNC; HGNC:2514; CTNNB1; Simple; p.Val79Ile (c.235G>A); Zygosity=Unspecified (PubMed=24755471).
CC   Sequence variation: Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Unspecified (PubMed=20570890; PubMed=24755471).
CC   Sequence variation: Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Unspecified (PubMed=20570890; PubMed=24755471).
CC   Sequence variation: Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Asp549Asn (c.1645G>A); ClinVar=VCV000375900; Zygosity=Unspecified (PubMed=20570890; PubMed=24755471).
CC   Sequence variation: Mutation; HGNC; HGNC:6768; SMAD2; Simple; p.Pro164fs*8 (c.490_491insA); Zygosity=Unspecified (PubMed=24755471).
CC   Sequence variation: Mutation; HGNC; HGNC:6769; SMAD3; Simple; p.Val244Ile (c.730G>A); ClinVar=VCV000919044; Zygosity=Unspecified (PubMed=24755471).
CC   Omics: Glycomics; N-glycan profiling.
CC   Omics: Proteomics; Expression; Reverse-phase protein array.
CC   Omics: Transcriptomics; Microarray.
CC   Omics: Transcriptomics; RNAseq.
CC   Omics: Variations; SNP array analysis.
CC   Misspelling: HCT 18; Note=Occasionally.
CC   Derived from site: In situ; Colon; UBERON=UBERON_0001155.
CC   Cell type: Epithelial cell of colon; CL=CL_0011108.
ST   Source(s): AddexBio=C0009017/4907; CCRID; CLS=300210; KCLB=10244; PubMed=25877200; PubMed=25926053
ST   Amelogenin: X (CCRID; PubMed=25877200)
ST   Amelogenin: X,Y (AddexBio=C0009017/4907; CLS=300210; KCLB=10244; PubMed=25926053)
ST   CSF1PO: 12
ST   D13S317: 8,11
ST   D16S539: 12,13
ST   D18S51: 11,17
ST   D19S433: 14,16
ST   D21S11: 29,32.2
ST   D2S1338: 17,25
ST   D3S1358: 17
ST   D5S818: 13
ST   D7S820: 10,11.3 (CCRID; PubMed=25877200)
ST   D7S820: 10,11.3,12 (AddexBio=C0009017/4907)
ST   D7S820: 10,12 (CLS=300210; KCLB=10244; PubMed=25926053)
ST   D8S1179: 15
ST   FGA: 22
ST   Penta D: 9,14
ST   Penta E: 7,14
ST   TH01: 7,9.3
ST   TPOX: 8,11
ST   vWA: 18,19
DI   NCIt; C4349; Colon adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_3449 ! __Parent_cell_line_of_DLD-1/HCT 8/HCT 15/HRT-18
SX   Male
AG   67Y
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 10-04-25; Version: 41
CH   CVCL_5679 ! HCT-8/E11
CH   CVCL_5945 ! HCT-8/R1
CH   CVCL_5947 ! HCT-8/S10
CH   CVCL_5682 ! HCT-8/S11
CH   CVCL_A9CF ! HCT-8/VCR
CH   CVCL_A9CQ ! HCT-8-Luc2-tdT
CH   CVCL_A9CR ! HCT-8-mCherry
CH   CVCL_A9CS ! HCT-8-tdT
CH   CVCL_S851 ! HCT-8R
//
RX   PubMed=24755471; DOI=10.1158/0008-5472.CAN-14-0013;
RA   Mouradov, Dmitri
RA   Sloggett, Clare
RA   Jorissen, Robert N.
RA   Love, Christopher G.
RA   Li, Shan
RA   Burgess, Antony Wilks
RA   Arango, Diego
RA   Strausberg, Robert L.
RA   Buchanan, Daniel
RA   Wormald, Samuel
RA   O'Connor, Liam
RA   Wilding, Jennifer L.
RA   Bicknell, David C.
RA   Tomlinson, Ian Philip Mark
RA   Bodmer, Walter Fred
RA   Mariadason, John M.
RA   Sieber, Oliver M.
RT   "Colorectal cancer cell lines are representative models of the main
RT   molecular subtypes of primary cancer.";
RL   Cancer Res. 74:3238-3247(2014).
//
RX   PubMed=25877200; DOI=10.1038/nature14397;
RA   Yu, Ma-Mie
RA   Selvaraj, Suresh K.
RA   Liang-Chu, May M.Y.
RA   Aghajani, Sahar
RA   Busse, Matthew
RA   Yuan, Jean
RA   Lee, Genee
RA   Peale, Franklin V.
RA   Klijn, Christiaan
RA   Bourgon, Richard
RA   Kaminker, Joshua S.
RA   Neve, Richard M.
RT   "A resource for cell line authentication, annotation and quality
RT   control.";
RL   Nature 520:307-311(2015).
//
RX   PubMed=25485619; DOI=10.1038/nbt.3080;
RA   Klijn, Christiaan
RA   Durinck, Steffen
RA   Stawiski, Eric W.
RA   Haverty, Peter M.
RA   Jiang, Zhao-Shi
RA   Liu, Han-Bin
RA   Degenhardt, Jeremiah
RA   Mayba, Oleg
RA   Gnad, Florian
RA   Liu, Jin-Feng
RA   Pau, Gregoire
RA   Reeder, Jens
RA   Cao, Yi
RA   Mukhyala, Kiran
RA   Selvaraj, Suresh K.
RA   Yu, Ma-Mie
RA   Zynda, Gregory J.
RA   Brauer, Matthew J.
RA   Wu, Thomas D.
RA   Gentleman, Robert Clifford
RA   Manning, Gerard
RA   Yauch, Robert L.
RA   Bourgon, Richard
RA   Stokoe, David
RA   Modrusan, Zora
RA   Neve, Richard M.
RA   de Sauvage, Frederic J.
RA   Settleman, Jeffrey
RA   Seshagiri, Somasekar
RA   Zhang, Ze-Min
RT   "A comprehensive transcriptional portrait of human cancer cell
RT   lines.";
RL   Nat. Biotechnol. 33:306-312(2015).
//
RX   PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103;
RA   Ghandi, Mahmoud
RA   Huang, Franklin W.
RA   Jane-Valbuena, Judit
RA   Kryukov, Gregory V.
RA   Lo, Christopher C.
RA   McDonald, E. Robert 3rd
RA   Barretina, Jordi Ginesta
RA   Gelfand, Ellen T.
RA   Bielski, Craig M.
RA   Li, Hao-Xin
RA   Hu, Kevin
RA   Andreev-Drakhlin, Alexander Y.
RA   Kim, Jaegil
RA   Hess, Julian M.
RA   Haas, Brian J.
RA   Aguet, Francois
RA   Weir, Barbara A.
RA   Rothberg, Michael V.
RA   Paolella, Brenton R.
RA   Lawrence, Michael Scott
RA   Akbani, Rehan
RA   Lu, Yi-Ling
RA   Tiv, Hong L.
RA   Gokhale, Prafulla C.
RA   de Weck, Antoine
RA   Mansour, Ali Amin
RA   Oh, Coyin
RA   Shih, Juliann
RA   Hadi, Kevin
RA   Rosen, Yanay
RA   Bistline, Jonathan
RA   Venkatesan, Kavitha
RA   Reddy, Anupama
RA   Sonkin, Dmitriy
RA   Liu, Manway
RA   Lehar, Joseph
RA   Korn, Joshua M.
RA   Porter, Dale A.
RA   Jones, Michael D.
RA   Golji, Javad
RA   Caponigro, Giordano
RA   Taylor, Jordan E.
RA   Dunning, Caitlin M.
RA   Creech, Amanda L.
RA   Warren, Allison C.
RA   McFarland, James M.
RA   Zamanighomi, Mahdi
RA   Kauffmann, Audrey
RA   Stransky, Nicolas
RA   Imielinski, Marcin
RA   Maruvka, Yosef E.
RA   Cherniack, Andrew D.
RA   Tsherniak, Aviad
RA   Vazquez, Francisca
RA   Jaffe, Jacob D.
RA   Lane, Andrew Alan
RA   Weinstock, David M.
RA   Johannessen, Cory M.
RA   Morrissey, Michael P.
RA   Stegmeier, Frank
RA   Schlegel, Robert
RA   Hahn, William Chun
RA   Getz, Gad
RA   Mills, Gordon B.
RA   Boehm, Jesse S.
RA   Golub, Todd Robert
RA   Garraway, Levi Alexander
RA   Sellers, William Raj
RT   "Next-generation characterization of the Cancer Cell Line
RT   Encyclopedia.";
RL   Nature 569:503-508(2019).
//
RX   PubMed=33389257; DOI=10.1007/s10096-020-04106-0; PMCID=PMC7778494;
RA   Wurtz, Nathalie
RA   Penant, Gwilherm
RA   Jardot, Priscilla
RA   Duclos, Nathalie
RA   La Scola, Bernard
RT   "Culture of SARS-CoV-2 in a panel of laboratory cell lines,
RT   permissivity, and differences in growth profile.";
RL   Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021).
//
RX   PubMed=10674020; DOI=10.1016/S0959-8049(99)00206-3;
RA   Ku, Ja-Lok
RA   Yoon, Kyong-Ah
RA   Kim, Dae-Yong
RA   Park, Jae-Gahb
RT   "Mutations in hMSH6 alone are not sufficient to cause the
RT   microsatellite instability in colorectal cancer cell lines.";
RL   Eur. J. Cancer 35:1724-1729(1999).
//
RX   PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076;
RA   Li, Jun
RA   Zhao, Wei
RA   Akbani, Rehan
RA   Liu, Wen-Bin
RA   Ju, Zhen-Lin
RA   Ling, Shi-Yun
RA   Vellano, Christopher P.
RA   Roebuck, Paul
RA   Yu, Qing-Hua
RA   Eterovic, Agda Karina
RA   Byers, Lauren Averett
RA   Davies, Michael A.
RA   Deng, Wan-Leng
RA   Gopal, Y.N. Vashisht
RA   Chen, Guo
RA   von Euw, Erika Maria
RA   Slamon, Dennis Joseph
RA   Conklin, Dylan
RA   Heymach, John Victor
RA   Gazdar, Adi F.
RA   Minna, John D.
RA   Myers, Jeffrey N.
RA   Lu, Yi-Ling
RA   Mills, Gordon B.
RA   Liang, Han
RT   "Characterization of human cancer cell lines by reverse-phase protein
RT   arrays.";
RL   Cancer Cell 31:225-239(2017).
//
RX   PubMed=26537799; DOI=10.1074/mcp.M115.051235; PMCID=PMC4762531;
RA   Holst, Stephanie
RA   Deuss, Anna J.M.
RA   van Pelt, Gabi W.
RA   van Vliet, Sandra Johanna
RA   Garcia-Vallejo, Juan J.
RA   Koeleman, Carolien A.M.
RA   Deelder, Andre M.
RA   Mesker, Wilma E.
RA   Tollenaar, Robertus Alexandre Eduard Mattheus
RA   Rombouts, Yoann
RA   Wuhrer, Manfred
RT   "N-glycosylation profiling of colorectal cancer cell lines reveals
RT   association of fucosylation with differentiation and caudal type
RT   homebox 1 (CDX1)/villin mRNA expression.";
RL   Mol. Cell. Proteomics 15:124-140(2016).
//
RX   PubMed=20570890; DOI=10.1158/0008-5472.CAN-10-0192; PMCID=PMC2943514;
RA   Janakiraman, Manickam
RA   Vakiani, Efsevia
RA   Zeng, Zhao-Shi
RA   Pratilas, Christine A.
RA   Taylor, Barry Stephen
RA   Chitale, Dhananjay
RA   Halilovic, Ensar
RA   Wilson, Manda
RA   Huberman, Kety
RA   Ricarte Filho, Julio Cezar Marques
RA   Persaud, Yogindra
RA   Levine, Douglas A.
RA   Fagin, James Alexander
RA   Jhanwar, Suresh C.
RA   Mariadason, John M.
RA   Lash, Alex
RA   Ladanyi, Marc
RA   Saltz, Leonard B.
RA   Heguy, Adriana
RA   Paty, Philip B.
RA   Solit, David B.
RT   "Genomic and biological characterization of exon 4 KRAS mutations in
RT   human cancer.";
RL   Cancer Res. 70:5901-5911(2010).
//
RX   PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878;
RA   Scholtalbers, Jelle
RA   Boegel, Sebastian
RA   Bukur, Thomas
RA   Byl, Marius
RA   Goerges, Sebastian
RA   Sorn, Patrick
RA   Loewer, Martin
RA   Sahin, Ugur
RA   Castle, John C.
RT   "TCLP: an online cancer cell line catalogue integrating HLA type,
RT   predicted neo-epitopes, virus and gene expression.";
RL   Genome Med. 7:118.1-118.7(2015).
//
RX   PubMed=8422623; DOI=10.1002/1097-0142(19930115)71:2<315::AID-CNCR2820710208>3.0.CO;2-B;
RA   Tibbetts, Lance M.
RA   Doremus, Craig M.
RA   Tzanakakis, George N.
RA   Vezeridis, Michael P.
RT   "Liver metastases with 10 human colon carcinoma cell lines in nude
RT   mice and association with carcinoembryonic antigen production.";
RL   Cancer 71:315-321(1993).
//
RX   PubMed=25926053; DOI=10.1038/ncomms8002;
RA   Medico, Enzo
RA   Russo, Mariangela
RA   Picco, Gabriele
RA   Cancelliere, Carlotta
RA   Valtorta, Emanuele
RA   Corti, Giorgio
RA   Buscarino, Michela
RA   Isella, Claudio
RA   Lamba, Simona
RA   Martinoglio, Barbara
RA   Veronese, Silvio
RA   Siena, Salvatore
RA   Sartore-Bianchi, Andrea
RA   Beccuti, Marco
RA   Mottolese, Marcella
RA   Linnebacher, Michael
RA   Cordero, Francesca
RA   Di Nicolantonio, Federica
RA   Bardelli, Alberto
RT   "The molecular landscape of colorectal cancer cell lines unveils
RT   clinically actionable kinase targets.";
RL   Nat. Commun. 6:7002.1-7002.10(2015).
//
RX   PubMed=25944804; DOI=10.1158/1078-0432.CCR-14-2457;
RA   Bazzocco, Sarah
RA   Dopeso, Higinio
RA   Carton-Garcia, Fernando
RA   Macaya, Irati
RA   Andretta, Elena
RA   Chionh, Fiona
RA   Rodrigues, Paulo
RA   Garrido, Miriam
RA   Alazzouzi, Hafid
RA   Nieto, Rocio
RA   Sanchez, Alex
RA   Schwartz, Simo Jr.
RA   Bilic, Josipa
RA   Mariadason, John M.
RA   Arango, Diego
RT   "Highly expressed genes in rapidly proliferating tumor cells as new
RT   targets for colorectal cancer treatment.";
RL   Clin. Cancer Res. 21:3695-3704(2015).
//
RX   PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662;
RA   Rothenberg, S. Michael
RA   Mohapatra, Gayatry
RA   Rivera, Miguel N.
RA   Winokur, Daniel
RA   Greninger, Patricia
RA   Nitta, Mai
RA   Sadow, Peter M.
RA   Sooriyakumar, Gaya
RA   Brannigan, Brian W.
RA   Ulman, Matthew J.
RA   Perera, Rushika M.
RA   Wang, Rui
RA   Tam, Angela
RA   Ma, Xiao-Jun
RA   Erlander, Mark
RA   Sgroi, Dennis C.
RA   Rocco, James W.
RA   Lingen, Mark W.
RA   Cohen, Ezra E.W.
RA   Louis, David Neil
RA   Settleman, Jeffrey
RA   Haber, Daniel Arie
RT   "A genome-wide screen for microdeletions reveals disruption of
RT   polarity complex genes in diverse human cancers.";
RL   Cancer Res. 70:2158-2164(2010).
//
RX   PubMed=77569; DOI=10.1111/j.1399-0039.1978.tb01259.x;
RA   Espmark, Jons Ake
RA   Ahlqvist-Roth, Lena
RA   Sarne, Lena
RA   Persson, Anita
RT   "Tissue typing of cells in culture. III. HLA antigens of established
RT   human cell lines. Attempts at typing by the mixed hemadsorption
RT   technique.";
RL   Tissue Antigens 11:279-286(1978).
//
RX   PubMed=9809040; DOI=10.1016/S0165-4608(98)00081-8;
RA   Vermeulen, Stefan Joris
RA   Chen, Tchaw-Ren
RA   Speleman, Frank
RA   Nollet, Friedel
RA   Van Roy, Frans M.
RA   Mareel, Marc M.
RT   "Did the four human cancer cell lines DLD-1, HCT-15, HCT-8, and HRT-18
RT   originate from one and the same patient?";
RL   Cancer Genet. Cytogenet. 107:76-79(1998).
//