Cellosaurus logo
expasy logo

Cellosaurus JOSK-I (CVCL_2082)

[Text version]
Cell line name JOSK-I
Accession CVCL_2082
Resource Identification Initiative To cite this cell line use: JOSK-I (RRID:CVCL_2082)
Comments Problematic cell line: Contaminated. Shown to be a U-937 derivative (PubMed=10508494; PubMed=12592342; PubMed=20143388).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00135.
Population: Caucasian.
Doubling time: ~28-35 hours (DSMZ=ACC-155).
Misspelling: JOSK-1; Cosmic=947371.
Derived from site: In situ; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene fusion; HGNC; 16063; MLLT10 + HGNC; 15514; PICALM; Name(s)=PICALM-MLLT10, CALM-AF10 (from parent cell line).
  • Mutation; HGNC; 9588; PTEN; Simple; p.Gly129fs*51 (c.387_388insCGCC); Zygosity=Hemizygous (from parent cell line).
  • Mutation; HGNC; 9644; PTPN11; Simple; p.Gly60Arg (c.178G>C); ClinVar=VCV000372590; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; c.559+1G>A (p.Val173Trpfs*59); ClinVar=VCV000428908; Zygosity=Homozygous; Note=Splice donor mutation (from parent cell line).
  • Mutation; HGNC; 12796; WT1; Simple; p.Arg301Ter (c.901C>T) (p.Arg352Ter, c.1054C>T) (p.Arg374Ter, c.1120C>T); ClinVar=VCV000449416; Zygosity=Heterozygous (from parent cell line).
Disease Adult acute monocytic leukemia (NCIt: C8263)
Acute monoblastic/monocytic leukemia (ORDO: Orphanet_514)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0007 (U-937)
Sex of cell Male
Age at sampling 37Y
Category Cancer cell line
STR profile Source(s): DSMZ

Markers:
AmelogeninX
CSF1PO12
D2S133817,20
D3S135816
D5S81812
D7S8209,11
D8S117912,13
D13S31710,12
D16S53912
D18S5112,13
D19S43314,16
D21S1127,29
FGA22,25
Penta D12,13
Penta E13
TH016,9.3
TPOX8,11
vWA14,15

Run an STR similarity search on this cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx
Publications

PubMed=3486043
Ohta M., Furukawa Y., Ide C., Akiyama N., Utakoji T., Miura Y., Saito M.
Establishment and characterization of four human monocytoid leukemia cell lines (JOSK-I, -S, -M and -K) with capabilities of monocyte-macrophage lineage differentiation and constitutive production of interleukin 1.
Cancer Res. 46:3067-3074(1986)

PubMed=9510473; DOI=10.1111/j.1349-7006.1998.tb00476.x
Hosoya N., Hangaishi A., Ogawa S., Miyagawa K., Mitani K., Yazaki Y., Hirai H.
Frameshift mutations of the hMSH6 gene in human leukemia cell lines.
Jpn. J. Cancer Res. 89:33-39(1998)

PubMed=10508494; DOI=10.1002/(SICI)1097-0215(19991112)83:4<555::AID-IJC19>3.0.CO;2-2
MacLeod R.A.F., Dirks W.G., Matsuo Y., Kaufmann M., Milch H., Drexler H.G.
Widespread intraspecies cross-contamination of human tumor cell lines arising at source.
Int. J. Cancer 83:555-563(1999)

PubMed=12592342; DOI=10.1038/sj.leu.2402799
Drexler H.G., Dirks W.G., Matsuo Y., MacLeod R.A.F.
False leukemia-lymphoma cell lines: an update on over 500 cell lines.
Leukemia 17:416-426(2003)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

Cross-references
Cell line collections (Providers) DSMZ; ACC-155 - Discontinued
Cell line databases/resources CLO; CLO_0007034
CLDB; cl2951
cancercelllines; CVCL_2082
DSMZCellDive; ACC-155
Biological sample resources BioSample; SAMN03151980
Encyclopedic resources Wikidata; Q54899045
Polymorphism and mutation databases Cosmic; 787438
Cosmic; 919120
Cosmic; 947371
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number27