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Cellosaurus Sup-T1 Nef-ER 31 (CVCL_1H35)

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Cell line name Sup-T1 Nef-ER 31
Synonyms Nef-ER 31; Nef-ER #31; Nef-ER #31 Clone; Nef-ER Expressing Sup-T1 Cells (Clone 31)
Accession CVCL_1H35
Resource Identification Initiative To cite this cell line use: Sup-T1 Nef-ER 31 (RRID:CVCL_1H35)
Comments Population: Caucasian.
Characteristics: Expresses a chimeric Nef-estrogen receptor hormone-binding domain (Nef-ER) protein. Nef-ER is kept in an inactive state due to steric hindrance, and addition of the membrane-permeable drug 4-hydroxytamoxifen (4-HT), which binds to the ER domain, leads to inducible activation of Nef-ER within cells (PubMed=11134296).
Genetic integration: Method=Transfection/transduction; Gene=MGI; MGI:1352467; Esr1 (Note=With p.Met1_Asp484del and p.Ile486_Ile599del).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q90VU7; HIV-1 isolate NL4-3 nef.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Pleural effusion; UBERON=UBERON_0000175.
Cell type: T-cell; CL=CL_0000084.
Sequence variations
  • Mutation; HGNC; HGNC:3236; EGFR; Simple; p.Cys251Tyr (c.752G>A); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6342; KIT; Simple; p.Arg177His (c.530G>A); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7029; MET; Simple; p.Arg191Trp (c.571C>T); ClinVar=VCV000568557; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Glu545Asp (c.1635G>T); ClinVar=VCV000217293; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Glu970Lys (c.2908G>A); ClinVar=VCV001057635; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:9585; PTCH1; Simple; p.Arg682Cys (c.2044C>T); ClinVar=VCV000237466; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:15533; SPRY4; Simple; p.Arg25Trp (c.73C>T); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg267Leu (c.800G>T); ClinVar=VCV000852206; Zygosity=Heterozygous (from parent cell line).
Disease Childhood T lymphoblastic lymphoma (NCIt: C7210)
T-cell non-Hodgkin lymphoma (ORDO: Orphanet_171918)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1714 (SUP-T1)
Sex of cell Male
Age at sampling 8Y
Category Cancer cell line
Publications

PubMed=11134296; DOI=10.1128/JVI.75.2.834-843.2001; PMCID=PMC113979
Walk S.F., Alexander M., Maier B., Hammarskjold M.-L., Rekosh D.M., Ravichandran K.S.
Design and use of an inducibly activated human immunodeficiency virus type 1 Nef to study immune modulation.
J. Virol. 75:834-843(2001)

Cross-references
Cell line collections (Providers) BEI_Resources; ARP-6453
Cell line databases/resources cancercelllines; CVCL_1H35
Encyclopedic resources Wikidata; Q54930388
Entry history
Entry creation08-Jul-2015
Last entry update19-Dec-2024
Version number16