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Cellosaurus MCF7 AREc32 (CVCL_1D32)

[Text version]
Cell line name MCF7 AREc32
Synonyms MCF7/AREc32; AREc32
Accession CVCL_1D32
Resource Identification Initiative To cite this cell line use: MCF7 AREc32 (RRID:CVCL_1D32)
Comments Population: Caucasian.
Characteristics: Transfected with a construct containing 8 copies of the rat glutathione-S-transferase (GST) antioxidant response element (ARE) linked to the luciferase reporter gene. Induction of the ARE results in luciferase activity (PubMed=17108137).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P08659; Firefly luciferase.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:4172; GATA3; Simple; p.Asp336Glyfs*17 (c.1006dupG); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Heterozygous (from parent cell line).
Disease Invasive breast carcinoma of no special type (NCIt: C4194)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0031 (MCF-7)
Sex of cell Female
Age at sampling 69Y
Category Cancer cell line
STR profile Source(s): ECACC=16071902

Markers:
AmelogeninX
CSF1PO10
D3S135816
D5S81811,12
D7S8209
D8S117910,14
D13S31711
D16S53911,12
D18S5114
D21S1130
FGA23,25
Penta D12
Penta E7,12
TH016
TPOX9,12
vWA14,15

Run an STR similarity search on this cell line
Web pages http://www.cxrbiosciences.com/technologies/arec32-cell-line/
Publications

PubMed=17108137; DOI=10.1158/0008-5472.CAN-06-2298
Wang X.-J., Hayes J.D., Wolf C.R.
Generation of a stable antioxidant response element-driven reporter gene cell line and its use to show redox-dependent activation of nrf2 by cancer chemotherapeutic agents.
Cancer Res. 66:10983-10994(2006)

PubMed=23032559; DOI=10.1039/c2em30506b
Escher B.I., Dutt M., Maylin E., Tang J.Y.M., Toze S., Wolf C.R., Lang M.
Water quality assessment using the AREc32 reporter gene assay indicative of the oxidative stress response pathway.
J. Environ. Monit. 14:2877-2885(2012)

Cross-references
Cell line collections (Providers) ABM; T6103
CancerTools; 151014
ECACC; 16071902 - Discontinued
Ximbio; 151014
Cell line databases/resources cancercelllines; CVCL_1D32
Encyclopedic resources Wikidata; Q54750439
Entry history
Entry creation08-Jul-2015
Last entry update19-Dec-2024
Version number20