Cellosaurus S2R+-Fucci (CVCL_1B58)
Cell line name | S2R+-Fucci |
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Synonyms | S2R+-FUCCI; S2R+_FUCCI |
Accession | CVCL_1B58 |
Resource Identification Initiative | To cite this cell line use: S2R+-Fucci (RRID:CVCL_1B58) |
Comments | Group: Insect cell line. Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci (Fluorescent Ubiquitination-based Cell Cycle Indicator) construct. Characteristics: Transfected with a Fucci like probe which consist of a nuclear eGFP fused to an E2F1 degron and unlocalized mRFP1 fused to a CycB degron. Doubling time: ~35 hours (DGRC=243). Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transfection/transduction; Gene=FPbase; 5YCFA; mRFP1 (Note=Bright red fluorescent protein derived from drFP583 (DsRed)). Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922. |
Species of origin | Drosophila melanogaster (Fruit fly)
(NCBI Taxonomy: 7227)
Breed/subspecies: Oregon-R; FlyBase_Strain=FBsn0000276. |
Hierarchy | Parent: CVCL_Z831 (S2R+) |
Sex of cell | Male |
Age at sampling | Late embryonic stage |
Category | Spontaneously immortalized cell line |
Cross-references | |
Cell line collections (Providers) | DGRC; 243 |
Cell line databases/resources | FlyBase_Cell_line; FBtc0000243 |
Encyclopedic resources | Wikidata; Q54951887 |
Entry history | |
Entry creation | 08-Jul-2015 |
Last entry update | 10-Sep-2024 |
Version number | 10 |