ID   C-433
AC   CVCL_1969
SY   C433
DR   CLO; CLO_0002127
DR   CLDB; cl520
DR   ATCC; CRL-2085
DR   BioSample; SAMN03151903
DR   BioSample; SAMN03471441
DR   cancercelllines; CVCL_1969
DR   DSMZ; ACC-268
DR   DSMZCellDive; ACC-268
DR   Wikidata; Q54799749
RX   PubMed=8222432;
CC   Problematic cell line: Contaminated. Shown to be a RD-ES derivative (DSMZ=ACC-268). Originally thought to originate from a 35 year old female patient with a benign giant cell tumor of the right distal tibia.
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00395.
CC   Population: Caucasian.
CC   Doubling time: ~25-30 hours (DSMZ=ACC-268).
CC   Sequence variation: Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Gene fusion; HGNC; 3508; EWSR1 + HGNC; 3749; FLI1; Name(s)=EWSR1-FLI1, EWS-FLI1; Note=Type 2 fusion (Ex7/Ex5) (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Arg273Cys (c.817C>T); ClinVar=VCV000043594; Zygosity=Unspecified (from parent cell line).
CC   Discontinued: ATCC; CRL-2085; true.
CC   Discontinued: DSMZ; ACC-268; true.
CC   Derived from site: In situ; Bone, humerus; UBERON=UBERON_0000976.
ST   Source(s): ATCC; DSMZ
ST   Amelogenin: X,Y
ST   CSF1PO: 11
ST   D13S317: 11
ST   D16S539: 11
ST   D18S51: 14,17.3
ST   D19S433: 13,14
ST   D21S11: 28
ST   D2S1338: 19
ST   D3S1358: 15
ST   D5S818: 11
ST   D7S820: 10
ST   D8S1179: 13
ST   FGA: 25
ST   Penta D: 9,12
ST   Penta E: 11
ST   TH01: 7
ST   TPOX: 9,11
ST   vWA: 17
DI   NCIt; C4817; Ewing sarcoma
DI   ORDO; Orphanet_319; Ewing sarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_2169 ! RD-ES
SX   Male
AG   19Y
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 30-01-24; Version: 33
RX   PubMed=8222432; DOI=10.1097/00003086-199311000-00039;
RA   Oreffo R.O.C., Marshall G.J., Kirchen M., Garcia C., Gallwitz W.E.,
RA   Chavez J., Mundy G.R., Bonewald L.F.;
RT   "Characterization of a cell line derived from a human giant cell tumor
RT   that stimulates osteoclastic bone resorption.";
RL   Clin. Orthop. Relat. Res. 296:229-241(1993).