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Cellosaurus MDA-N (CVCL_1910)

[Text version]
Cell line name MDA-N
Synonyms MDAN
Accession CVCL_1910
Resource Identification Initiative To cite this cell line use: MDA-N (RRID:CVCL_1910)
Comments Problematic cell line: Contaminated. Parent cell line (MDA-MB-435) has been shown to be a M14 derivative (PubMed=20143388).
Part of: NCI-60 cancer cell line panel.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00236.
Population: Caucasian.
Doubling time: 22.5 hours (NCI-DTP).
Microsatellite instability: Stable (MSS) (PubMed=12661003).
Omics: Array-based CGH.
Omics: CNV analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Derived from site: Metastatic; Right buttock, hypodermis; UBERON=UBERON_0013691+UBERON_0002072.
Sequence variations
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; c.150+2T>C (IVS1+2T>C); ClinVar=VCV000406712; Zygosity=Heterozygous; Note=Splice donor mutation (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; c.455insCdel26; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Gly266Glu (c.797G>A); ClinVar=VCV000161516; Zygosity=Heterozygous (from parent cell line).
Disease Amelanotic melanoma (NCIt: C3802)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0417 (MDA-MB-435)
Sex of cell Male
Age at sampling 33Y
Category Cancer cell line
STR profile Source(s): PubMed=19372543

Markers:
AmelogeninX
CSF1PO11
D2S133819,24
D3S135814
D5S81811,12
D7S8208,10
D8S117913
D13S31712
D16S53913
D18S5113,17
D19S43314
D21S1130
FGA21
TH016,7
TPOX8,11
vWA16,18

Run an STR similarity search on this cell line
Web pages https://dtp.cancer.gov/discovery_development/nci-60/cell_list.htm
https://dtp.cancer.gov/discovery_development/nci-60/mda-mb-435.htm
https://strap.nci.nih.gov/celline_detail.php?sample_id=52
https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

PubMed=10700174; DOI=10.1038/73432
Ross D.T., Scherf U., Eisen M.B., Perou C.M., Rees C., Spellman P.T., Iyer V.R., Jeffrey S.S., van de Rijn M., Waltham M.C., Pergamenschikov A., Lee J.C.F., Lashkari D., Shalon D., Myers T.G., Weinstein J.N., Botstein D., Brown P.O.
Systematic variation in gene expression patterns in human cancer cell lines.
Nat. Genet. 24:227-235(2000)

PubMed=12661003; DOI=10.1002/gcc.10196
Seitz S., Wassmuth P., Plaschke J., Schackert H.K., Karsten U., Santibanez-Koref M.F., Schlag P.M., Scherneck S.
Identification of microsatellite instability and mismatch repair gene mutations in breast cancer cell lines.
Genes Chromosomes Cancer 37:29-35(2003)

PubMed=19372543; DOI=10.1158/1535-7163.MCT-08-0921; PMCID=PMC4020356
Lorenzi P.L., Reinhold W.C., Varma S., Hutchinson A.A., Pommier Y., Chanock S.J., Weinstein J.N.
DNA fingerprinting of the NCI-60 cell line panel.
Mol. Cancer Ther. 8:713-724(2009)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=22347499; DOI=10.1371/journal.pone.0031628; PMCID=PMC3276511
Ruan X.-Y., Kocher J.-P.A., Pommier Y., Liu H.-F., Reinhold W.C.
Mass homozygotes accumulation in the NCI-60 cancer cell lines as compared to HapMap trios, and relation to fragile site location.
PLoS ONE 7:E31628-E31628(2012)

PubMed=24670534; DOI=10.1371/journal.pone.0092047; PMCID=PMC3966786
Varma S., Pommier Y., Sunshine M., Weinstein J.N., Reinhold W.C.
High resolution copy number variation data in the NCI-60 cancer cell lines from whole genome microarrays accessible through CellMiner.
PLoS ONE 9:E92047-E92047(2014)

Cross-references
Cell line databases/resources cancercelllines; CVCL_1910
Biological sample resources BioSample; SAMN03151833
Chemistry resources ChEMBL-Cells; CHEMBL3307532
ChEMBL-Targets; CHEMBL614078
PubChem_Cell_line; CVCL_1910
Encyclopedic resources Wikidata; Q54904649
Gene expression databases GEO; GSM2096
GEO; GSM750823
GEO; GSM799355
GEO; GSM799418
GEO; GSM1153425
GEO; GSM1178406
GEO; GSM1178407
GEO; GSM1181357
GEO; GSM1181358
Polymorphism and mutation databases Cosmic; 875880
Cosmic; 1998491
Progenetix; CVCL_1910
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number35