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Cellosaurus Mel JuSo (CVCL_1403)

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Cell line name Mel JuSo
Synonyms MEL-Juso; MEL-JUSO; Mel-Juso; Mel Juso; MelJuSo; MELJUSO; JuSo
Accession CVCL_1403
Resource Identification Initiative To cite this cell line use: Mel JuSo (RRID:CVCL_1403)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Caucasian.
Doubling time: ~28 hours (DSMZ=ACC-74).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Skin; UBERON=UBERON_0002097.
Sequence variations
  • Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (PubMed=9598804).
  • Gene deletion; HGNC; 1788; CDKN2B; Zygosity=Homozygous (PubMed=9598804).
  • Mutation; HGNC; 5173; HRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012604; Zygosity=Homozygous (Cosmic-CLP; DepMap).
  • Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (PubMed=10766161; PubMed=15009714; Cosmic-CLP; DepMap).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*01:01,01:01
HLA-BB*08:01,08:01
HLA-CC*07:01,07:01
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0
East Asian, North0
East Asian, South1.12
South Asian0
European, North63.28
European, South35.59
Disease Cutaneous melanoma (NCIt: C3510)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_B7EF (Mel JuSo ABCB5 KO clone HO1)CVCL_B7EG (Mel JuSo ABCB5 KO clone HO2)CVCL_B7EH (Mel JuSo ABCB5 KO clone HO3)
CVCL_B7EI (Mel JuSo ABCB5 KO clone HO4)
Sex of cell Female
Age at sampling 58Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP; DSMZ; PubMed=25877200

Markers:
AmelogeninX
CSF1PO10,11
D2S133820,24
D3S135814,17
D5S81811,12
D7S82010,12
D8S11799,14
D13S3178,9
D16S53912,13
D18S5115,18
D19S43315
D21S1129
FGA22
Penta D13
Penta E5,13
TH019.3
TPOX8,10
vWA16

Run an STR similarity search on this cell line
Publications

PubMed=6895502; DOI=10.1002/eji.1830111015
Johnson J.P., Demmer-Dieckmann M., Meo T., Hadam M.R., Riethmuller G.
Surface antigens of human melanoma cells defined by monoclonal antibodies. I. Biochemical characterization of two antigens found on cell lines and fresh tumors of diverse tissue origin.
Eur. J. Immunol. 11:825-831(1981)

PubMed=2174414; DOI=10.1002/ijc.2910460629
Deschodt-Lanckman M., Vanneste Y., Loir B., Michel A., Libert A., Ghanem G., Lejeune F.
Degradation of alpha-melanocyte stimulating hormone (alpha-MSH) by CALLA/endopeptidase 24.11 expressed by human melanoma cells in culture.
Int. J. Cancer 46:1124-1130(1990)

PubMed=9598804; DOI=10.1002/(SICI)1098-2264(199806)22:2<157::AID-GCC11>3.0.CO;2-N
Walker G.J., Flores J.F., Glendening J.M., Lin A.H.-T., Markl I.D.C., Fountain J.W.
Virtually 100% of melanoma cell lines harbor alterations at the DNA level within CDKN2A, CDKN2B, or one of their downstream targets.
Genes Chromosomes Cancer 22:157-163(1998)

PubMed=10766161
Tsao H., Zhang X., Fowlkes K., Haluska F.G.
Relative reciprocity of NRAS and PTEN/MMAC1 alterations in cutaneous melanoma cell lines.
Cancer Res. 60:1800-1804(2000)

PubMed=11668190; DOI=10.1177/002215540104901105
Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.
Immunocytochemical analysis of cell lines derived from solid tumors.
J. Histochem. Cytochem. 49:1369-1378(2001)

PubMed=15009714; DOI=10.1046/j.0022-202X.2004.22243.x
Tsao H., Goel V., Wu H., Yang G., Haluska F.G.
Genetic interaction between NRAS and BRAF mutations and PTEN/MMAC1 inactivation in melanoma.
J. Invest. Dermatol. 122:337-341(2004)

PubMed=18698037; DOI=10.1158/1078-0432.CCR-08-0186
Reschke M., Mihic-Probst D., van der Horst E.H., Knyazev P., Wild P.J., Hutterer M., Meyer S., Dummer R., Moch H., Ullrich A.
HER3 is a determinant for poor prognosis in melanoma.
Clin. Cancer Res. 14:5188-5197(2008)

PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

CLPUB00695
Guerit E.
Generation of an ABCB5-knockout human melanoma cell line using the CRISPR/Cas9 genome editing-tool: study of ABCB5 subcellular localization by cell fractionation.
Thesis MSc (2019), University of Namur, Belgium

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023
Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E.T., Schweppe D.K., Jedrychowski M.P., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) CLS; 300282 - Discontinued
DSMZ; ACC-74
Cell line databases/resources CLO; CLO_0007676
CLDB; cl3440
cancercelllines; CVCL_1403
Cell_Model_Passport; SIDM00336
Cosmic-CLP; 908125
DepMap; ACH-000881
DSMZCellDive; ACC-74
LINCS_LDP; LCL-1252
Anatomy/cell type resources BTO; BTO:0001588
Biological sample resources BioSample; SAMN03471884
BioSample; SAMN10988249
CRISP screens repositories BioGRID_ORCS_Cell_line; 548
Chemistry resources ChEMBL-Cells; CHEMBL3308761
ChEMBL-Targets; CHEMBL1075501
GDSC; 908125
PharmacoDB; MELJUSO_915_2019
PubChem_Cell_line; CVCL_1403
Encyclopedic resources Wikidata; Q54905064
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM887309
GEO; GSM888385
GEO; GSM1374670
GEO; GSM1670095
Polymorphism and mutation databases Cosmic; 706091
Cosmic; 888060
Cosmic; 888835
Cosmic; 897474
Cosmic; 908125
Cosmic; 1020272
Cosmic; 1022285
Cosmic; 1459306
Cosmic; 1995505
IARC_TP53; 21493
LiGeA; CCLE_674
Progenetix; CVCL_1403
Proteomic databases PRIDE; PXD011899
PRIDE; PXD019519
PRIDE; PXD021877
PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update30-Jan-2024
Version number40