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Cellosaurus HSC-4 (CVCL_1289)

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Cell line name HSC-4
Synonyms HSC4
Accession CVCL_1289
Resource Identification Initiative To cite this cell line use: HSC-4 (RRID:CVCL_1289)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Japanese.
Doubling time: ~25 hours (Note=Lots 04012002 and 06292012), ~21 hours (Note=Lot 03202018) (JCRB=JCRB0624).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: CRISPR phenotypic screen.
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: Metastatic; Cervical lymph node; UBERON=UBERON_0002429.
Sequence variations
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.Arg80Ter (c.238C>T) (p.Pro94Leu, c.281C>T); ClinVar=VCV000009409; Zygosity=Homozygous (DepMap=ACH-000546).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Heterozygous (PubMed=18097548; DepMap=ACH-000546).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (PubMed=31068700).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg248Gln (c.743G>A); ClinVar=VCV000012356; Zygosity=Unspecified (PubMed=1570156; PubMed=10741724).
HLA typing Source: PubMed=9023415
Class I
HLA-AA*24:02,31:01
HLA-BB*07:02,51:01
HLA-CC*07:02,14:02

Source: PubMed=26589293
Class I
HLA-AA*24:02,31:01
HLA-BB*07:02,51:01
HLA-CC*07:02,14:02
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0
East Asian, North84.74
East Asian, South13.79
South Asian1.33
European, North0
European, South0.12
Disease Tongue squamous cell carcinoma (NCIt: C4648)
Squamous cell carcinoma of the oral tongue (ORDO: Orphanet_457252)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Male
Age at sampling 64Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=907062; JCRB=JCRB0624; PubMed=11416159; RCB=RCB1902; Technion Genomics Center; TKG=TKG 0489

Markers:
AmelogeninX
CSF1PO9 (TKG=TKG 0489)
9,10 (Cosmic-CLP=907062; JCRB=JCRB0624; RCB=RCB1902; Technion Genomics Center)
D1S165615,16
D2S44110
D2S133824
D3S135815,16
D5S8188,11
D7S8209,10
D8S117913
D10S124812,13
D12S39118,19
D13S3178,13
D16S53912
D18S5115,20
D19S43313,14
D21S1129,30
D22S104517
FGA23
Penta D12
Penta E17,19
TH016,9
TPOX11
vWA16,19

Run an STR similarity search on this cell line
Publications

PubMed=2585303; DOI=10.1111/j.1600-0714.1989.tb01570.x
Momose F., Araida T., Negishi A., Ichijo H., Shioda S., Sasaki S.
Variant sublines with different metastatic potentials selected in nude mice from human oral squamous cell carcinomas.
J. Oral Pathol. Med. 18:391-395(1989)

PubMed=2228902; DOI=10.1007/BF02624609
Rikimaru K., Toda H., Tachikawa N., Kamata N., Enomoto S.
Growth of the malignant and nonmalignant human squamous cells in a protein-free defined medium.
In Vitro Cell. Dev. Biol. 26:849-856(1990)

PubMed=1570156
Sakai E., Tsuchida N.
Most human squamous cell carcinomas in the oral cavity contain mutated p53 tumor-suppressor genes.
Oncogene 7:927-933(1992)

PubMed=7591301; DOI=10.1002/ijc.2910640504
Eura M., Ogi K., Chikamatsu K., Lee K.D., Nakano K., Masuyama K., Itoh K., Ishikawa T.
Expression of the MAGE gene family in human head-and-neck squamous-cell carcinomas.
Int. J. Cancer 64:304-308(1995)

PubMed=9023415; DOI=10.1006/cimm.1996.1062
Seki N., Hoshino T., Kikuchi M., Hayashi A., Itoh K.
HLA-A locus-restricted and tumor-specific CTLs in tumor-infiltrating lymphocytes of patients with non-small cell lung cancer.
Cell. Immunol. 175:101-110(1997)

PubMed=9247707; DOI=10.1080/15216549700202901
Hatakeyama S., Gao Y.-H., Ohara-Nemoto Y., Kataoka H., Satoh M.
Expression of bone morphogenetic proteins of human neoplastic epithelial cells.
Biochem. Mol. Biol. Int. 42:497-505(1997)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=10069537; DOI=10.1111/j.1600-0714.1999.tb02006.x
Hoteiya T., Hayashi E., Satomura K., Kamata N., Nagayama M.
Expression of E-cadherin in oral cancer cell lines and its relationship to invasiveness in SCID mice in vivo.
J. Oral Pathol. Med. 28:107-111(1999)

PubMed=10741724
Eura M., Chikamatsu K., Katsura F., Obata A., Sobao Y., Takiguchi M., Song Y.-S., Appella E., Whiteside T.L., DeLeo A.B.
A wild-type sequence p53 peptide presented by HLA-A24 induces cytotoxic T lymphocytes that recognize squamous cell carcinomas of the head and neck.
Clin. Cancer Res. 6:979-986(2000)

PubMed=11416159; DOI=10.1073/pnas.121616198; PMCID=PMC35459
Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=11755821; DOI=10.1016/S1368-8375(01)00022-7
Niinaka Y., Haga A., Negishi A., Yoshimasu H., Raz A., Amagasa T.
Regulation of cell motility via high and low affinity autocrine motility factor (AMF) receptor in human oral squamous carcinoma cells.
Oral Oncol. 38:49-55(2002)

PubMed=12738951; DOI=10.1067/moe.2003.36
Sugiyama M., Bhawal U.K., Dohmen T., Ono S., Miyauchi M., Ishikawa T.
Detection of human papillomavirus-16 and HPV-18 DNA in normal, dysplastic, and malignant oral epithelium.
Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 95:594-600(2003)

PubMed=17052259; DOI=10.1111/j.1349-7006.2006.00343.x; PMCID=PMC11160012
Kozaki K.-i., Imoto I., Pimkhaokham A., Hasegawa S., Tsuda H., Omura K., Inazawa J.
PIK3CA mutation is an oncogenic aberration at advanced stages of oral squamous cell carcinoma.
Cancer Sci. 97:1351-1358(2006)

PubMed=17325662; DOI=10.1038/sj.onc.1210330
Nakaya K., Yamagata H.D., Arita N., Nakashiro K.-i., Nose M., Miki T., Hamakawa H.
Identification of homozygous deletions of tumor suppressor gene FAT in oral cancer using CGH-array.
Oncogene 26:5300-5308(2007)

PubMed=17599052; DOI=10.1038/sj.onc.1210589
Suzuki E., Imoto I., Pimkhaokham A., Nakagawa T., Kamata N., Kozaki K.-i., Amagasa T., Inazawa J.
PRTFDC1, a possible tumor-suppressor gene, is frequently silenced in oral squamous-cell carcinomas by aberrant promoter hypermethylation.
Oncogene 26:7921-7932(2007)

PubMed=18097548; DOI=10.3892/ijo.32.1.101
Murugan A.K., Hong N.T., Fukui Y., Munirajan A.K., Tsuchida N.
Oncogenic mutations of the PIK3CA gene in head and neck squamous cell carcinomas.
Int. J. Oncol. 32:101-111(2008)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Behan F.M., Iorio F., Picco G., Goncalves E., Beaver C.M., Migliardi G., Santos R., Rao Y., Sassi F., Pinnelli M., Ansari R., Harper S., Jackson D.A., McRae R., Pooley R., Wilkinson P., van der Meer D.J., Dow D., Buser-Doepner C.A., Bertotti A., Trusolino L., Stronach E.A., Saez-Rodriguez J., Yusa K., Garnett M.J.
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. 3rd, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023; PMCID=PMC7339254
Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. 3rd, Kalocsay M., Jane-Valbuena J., Gelfand E.T., Schweppe D.K., Jedrychowski M.P., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

PubMed=32990596; DOI=10.7554/eLife.57761; PMCID=PMC7591259
Chai A.W.-Y., Yee P.-S., Price S., Yee S.-M., Lee H.-M., Tiong V.K.H., Goncalves E., Behan F.M., Bateson J., Gilbert J.G.R., Tan A.-C., McDermott U., Garnett M.J., Cheong S.-C.
Genome-wide CRISPR screens of oral squamous cell carcinoma reveal fitness genes in the Hippo pathway.
eLife 9:e57761.1-e57761.34(2020)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

PubMed=37949965; DOI=10.1038/s41598-023-46926-y; PMCID=PMC10638439
Komune N., Sato K., Ono M., Imaizumi A., Masuda S., Itoyama S., Manako T., Kuga R., Hongo T., Kogo R., Onishi H., Nakagawa T.
Biological and genetic characterization of a newly established human external auditory canal carcinoma cell line, SCEACono2.
Sci. Rep. 13:19636-19636(2023)

Cross-references
Cell line collections (Providers) JCRB; JCRB0624
RCB; RCB1902
TKG; TKG 0489
Cell line databases/resources CLO; CLO_0051567
cancercelllines; CVCL_1289
Cell_Model_Passport; SIDM00588
CGH-DB; 365-2
Cosmic-CLP; 907062
DepMap; ACH-000546
LINCS_LDP; LCL-1218
Anatomy/cell type resources BTO; BTO:0002026
Biological sample resources BioSample; SAMN03471638
BioSample; SAMN03472086
BioSample; SAMN10987856
CRISP screens repositories BioGRID_ORCS_Cell_line; 892
Chemistry resources ChEMBL-Cells; CHEMBL3308109
ChEMBL-Targets; CHEMBL1075469
GDSC; 907062
PharmacoDB; HSC4_622_2019
PubChem_Cell_line; CVCL_1289
Encyclopedic resources Wikidata; Q54896390
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM827203
GEO; GSM850384
GEO; GSM887135
GEO; GSM888206
GEO; GSM1669906
GEO; GSM7695972
GEO; GSM7695973
Polymorphism and mutation databases Cosmic; 907062
Cosmic; 930332
Cosmic; 932149
Cosmic; 1046029
Cosmic; 1118790
Cosmic; 1120699
Cosmic; 1123040
Cosmic; 1140782
Cosmic; 1219866
Cosmic; 2266785
Cosmic; 2546837
IARC_TP53; 1102
LiGeA; CCLE_513
Progenetix; CVCL_1289
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Sep-2024
Version number42