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Cellosaurus Daoy (CVCL_1167)

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Cell line name Daoy
Synonyms DAOY; D324 Med; D-324 Med; D324 MED; D-324MED; D324
Accession CVCL_1167
Secondary accession CVCL_7903
Resource Identification Initiative To cite this cell line use: Daoy (RRID:CVCL_1167)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Caucasian.
Doubling time: 33.6 hours (Note=At 48th passage) (PubMed=2993532); 32 +- 1.5 hours (PubMed=31346954); 34 hours (ATCC=HTB-186).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: Proteome analysis by 2D-DE/MS.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Brain, posterior fossa; UBERON=UBERON_0008788.
Sequence variations
  • Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (ATCC=HTB-186).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (PubMed=31068700).
  • Mutation; HGNC; 11998; TP53; Simple; p.Cys242Phe (c.725G>T); ClinVar=VCV000376578; Zygosity=Homozygous (PubMed=22343310; ATCC=HTB-186; Cosmic-CLP=906833; DepMap=ACH-000211).
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.45
Native American0
East Asian, North1.54
East Asian, South0
South Asian1.1
European, North64.36
European, South32.54
Disease Medulloblastoma, SHH-activated, TP53-mutant (NCIt: C129442)
Medulloblastoma (ORDO: Orphanet_616)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_C8V5 (Daoy EZHIP KO)CVCL_A9DF (Daoy-luc2-tdT)
Sex of cell Male
Age at sampling 4Y
Category Cancer cell line
STR profile Source(s): ATCC=HTB-186; CCRID; Cosmic-CLP=906833; PubMed=25877200

Markers:
AmelogeninX
CSF1PO11
D2S133816,17
D3S135815
D5S81811,13
D7S8208,10
D8S117913,15
D13S31713,14
D16S53910
D18S5112
D19S43314,14.2 (ATCC=HTB-186; CCRID)
14.2 (PubMed=25877200)
D21S1129,31.2
FGA23
Penta D10,13
Penta E7,11
TH019
TPOX8,10
vWA14,20

Run an STR similarity search on this cell line
Web pages https://en.wikipedia.org/wiki/DAOY_(biology)
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/d/cell-lines-detail-566.html
Publications

PubMed=2993532; DOI=10.1097/00005072-198509000-00003
Jacobsen P.F., Jenkyn D.J., Papadimitriou J.M.
Establishment of a human medulloblastoma cell line and its heterotransplantation into nude mice.
J. Neuropathol. Exp. Neurol. 44:472-485(1985)

PubMed=1822845
Wikstrand C.J., Friedman H.S., Bigner D.D.
Medulloblastoma cell-substrate interaction in vitro.
Invasion Metastasis 11:310-324(1991)

DOI=10.1016/B978-0-12-333530-2.50005-8
Nister M., Westermark B.
Human glioma cell lines.
(In book chapter) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.17-42; Academic Press; New York; USA (1994)

DOI=10.1007/0-306-46861-1_11
Ali-Osman F.
Brain tumors.
(In book chapter) Human cell culture. Vol. 2. Cancer cell lines part 2; Masters J.R.W., Palsson B.O. (eds.); pp.167-184; Kluwer Academic Publishers; New York; USA (1999)

PubMed=12973738; DOI=10.1002/pmic.200300460
Peyrl A., Krapfenbauer K., Slavc I., Yang J.-W., Strobel T., Lubec G.
Protein profiles of medulloblastoma cell lines DAOY and D283: identification of tumor-related proteins and principles.
Proteomics 3:1781-1800(2003)

PubMed=18773455; DOI=10.1002/cyto.a.20633
Srivastava V.K., Nalbantoglu J.
Flow cytometric characterization of the DAOY medulloblastoma cell line for the cancer stem-like phenotype.
Cytometry A 73:940-948(2008)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20847082; DOI=10.1093/neuonc/noq091; PMCID=PMC3018940
Teider N., Scott D.K., Neiss A., Weeraratne S.D., Amani V.M., Wang Y.-F., Marquez V.E., Cho Y.-J., Pomeroy S.L.
Neuralized1 causes apoptosis and downregulates Notch target genes in medulloblastoma.
Neuro-oncol. 12:1244-1256(2010)

PubMed=22343310; DOI=10.1016/j.neulet.2012.02.022
Ghassemifar S., Mendrysa S.M.
MDM2 antagonism by nutlin-3 induces death in human medulloblastoma cells.
Neurosci. Lett. 513:106-110(2012)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=22723427; DOI=10.1093/neuonc/nos130; PMCID=PMC3424207
Sikkema A.H., den Dunnen W.F.A., Hulleman E., van Vuurden D.G., Garcia-Manero G., Yang H., Scherpen F.J.G., Kampen K.R., Hoving E.W., Kamps W.A., Diks S.H., Peppelenbosch M.P., de Bont E.S.J.M.
EphB2 activity plays a pivotal role in pediatric medulloblastoma cell adhesion and invasion.
Neuro-oncol. 14:1125-1135(2012)

PubMed=24297863; DOI=10.1158/1078-0432.CCR-13-2281; PMCID=PMC4198154
Bandopadhayay P., Bergthold G., Nguyen B., Schubert S., Gholamin S., Tang Y.-J., Bolin S., Schumacher S.E., Zeid R., Masoud S., Yu F.-R., Vue N., Gibson W.J., Paolella B.R., Mitra S.S., Cheshier S.H., Qi J., Liu K.-W., Wechsler-Reya R.J., Weiss W.A., Swartling F.J., Kieran M.W., Bradner J.E., Beroukhim R., Cho Y.-J.
BET bromodomain inhibition of MYC-amplified medulloblastoma.
Clin. Cancer Res. 20:912-925(2014)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=27498314; DOI=10.1016/j.jbiotec.2016.07.028
Ivanov D.P., Coyle B., Walker D.A., Grabowska A.M.
In vitro models of medulloblastoma: choosing the right tool for the job.
J. Biotechnol. 236:10-25(2016)

PubMed=27812533; DOI=10.1016/j.dib.2016.10.004; PMCID=PMC5079239
Ivanov D.P., Walker D.A., Coyle B., Grabowska A.M.
Data on the number and frequency of scientific literature citations for established medulloblastoma cell lines.
Data Brief 9:696-698(2016)

PubMed=29900115; DOI=10.1016/j.euprot.2016.03.015; PMCID=PMC5988510
Martelli C., D'Angelo L., Barba M., Baranzini M., Inserra I., Iavarone F., Vincenzoni F., Tamburrini G., Massimi L., Di Rocco C., Caldarelli M., Messana I., Michetti F., Castagnola M., Lattanzi W., Desiderio C.
Top-down proteomic characterization of DAOY medulloblastoma tumor cell line.
EuPA Open Proteom. 12:13-21(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. 3rd, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31346954; DOI=10.1007/s10616-019-00332-3; PMCID=PMC6787134
Bonfim Silva R., Salomao K.B., de Andrade Pimentel T.V.C., de Oliveira Menezes C.C.B., Palma P.V.B., Fontes A.M.
Biological characterization of the UW402, UW473, ONS-76 and DAOY pediatric medulloblastoma cell lines.
Cytotechnology 71:893-903(2019)

PubMed=31963405; DOI=10.3390/cancers12010226; PMCID=PMC7016648
Casciati A., Tanori M., Manczak R., Saada S., Tanno B., Giardullo P., Porcu E., Rampazzo E., Persano L., Viola G., Dalmay C., Lalloue F., Pothier A., Merla C., Mancuso M.
Human medulloblastoma cell lines: investigating on cancer stem cell-like phenotype.
Cancers (Basel) 12:226.1-226.14(2020)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023; PMCID=PMC7339254
Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. 3rd, Kalocsay M., Jane-Valbuena J., Gelfand E.T., Schweppe D.K., Jedrychowski M.P., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ATCC; HTB-186
BCRJ; 0325
CLS; 305053
KCB; KCB 2010203YJ
NCBI_Iran; C519
Cell line databases/resources CLO; CLO_0002699
cancercelllines; CVCL_1167
CCRID; 1101HUM-PUMC000218
CCRID; 3101HUMSCSP509
Cell_Model_Passport; SIDM00887
Cosmic-CLP; 906833
DepMap; ACH-000211
LINCS_LDP; LCL-1576
Lonza; 1438
Anatomy/cell type resources BTO; BTO:0004328
Biological sample resources BioSample; SAMN03470909
BioSample; SAMN10988097
ENCODE; ENCBS055ILX
ENCODE; ENCBS812KQP
CRISP screens repositories BioGRID_ORCS_Cell_line; 687
Chemistry resources ChEMBL-Cells; CHEMBL3308510
ChEMBL-Targets; CHEMBL612519
GDSC; 906833
PharmacoDB; Daoy_282_2019
PubChem_Cell_line; CVCL_1167
Encyclopedic resources Wikidata; Q29511023
Experimental variables resources EFO; EFO_0005698
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM482332
GEO; GSM886972
GEO; GSM888041
GEO; GSM919361
GEO; GSM1669725
GEO; GSM1938719
Polymorphism and mutation databases Cosmic; 687773
Cosmic; 906833
Cosmic; 923654
Cosmic; 973262
Cosmic; 974281
Cosmic; 975027
Cosmic; 1404441
Cosmic; 1995383
Cosmic; 2362649
Cosmic; 2731177
IARC_TP53; 480
LiGeA; CCLE_339
Progenetix; CVCL_1167
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number43