ID   UROtsa
AC   CVCL_0571
DR   BTO; BTO:0005004
DR   MCCL; MCC:0000476
DR   Cosmic; 867840
DR   ENCODE; ENCBS268AAA
DR   ENCODE; ENCBS269AAA
DR   GEO; GSM659708
DR   GEO; GSM659709
DR   GEO; GSM659710
DR   Wikidata; Q28872803
RX   PubMed=8788275;
RX   PubMed=11564615;
RX   PubMed=19921857;
RX   PubMed=20186695;
RX   PubMed=23691160;
RX   PubMed=26783756;
RX   PubMed=30550540;
WW   https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx
WW   http://genome.ucsc.edu/ENCODE/protocols/cell/human/Urothelia_Crawford_protocol.pdf
WW   https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/u/cell-lines-detail-540.html
CC   Problematic cell line: Partially contaminated. Some stocks of UROTsa are contaminated with T24 (PubMed=23691160). However it must be noted that the stocks from the laboratory distributing/curating this cell line are not contaminated; therefore the contamination event has probably occurred through secondary sources after UROtsa was given to other investigators (PubMed=26783756).
CC   Part of: ENCODE project common cell types; tier 3.
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00484.
CC   Doubling time: 29.7 hours (PubMed=8788275); 33.2 +- 0.8 hours (PubMed=19921857; PubMed=20186695).
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
CC   Omics: Transcriptome analysis by microarray.
CC   Derived from site: In situ; Urinary bladder, urothelium; UBERON=UBERON_0004645.
CC   Cell type: Urothelial cell; CL=CL_0000731.
ST   Source(s): PubMed=23691160; PubMed=26783756
ST   Amelogenin: X
ST   CSF1PO: 11,12
ST   D13S317: 11,13
ST   D16S539: 11,12
ST   D18S51: 16
ST   D21S11: 27,29
ST   D3S1358: 15
ST   D5S818: 12,13
ST   D7S820: 11,12
ST   D8S1179: 14
ST   FGA: 20
ST   Penta D: 11,12
ST   Penta E: 11,17
ST   TH01: 6,9.3
ST   TPOX: 8,9
ST   vWA: 16
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Female
AG   12Y
CA   Transformed cell line
DT   Created: 04-04-12; Last updated: 29-06-23; Version: 22
//
RX   PubMed=8788275; DOI=10.1007/BF00698738;
RA   Petzoldt J.L., Leigh I.M., Duffy P.G., Sexton C.J., Masters J.R.W.;
RT   "Immortalisation of human urothelial cells.";
RL   Urol. Res. 23:377-380(1995).
//
RX   PubMed=11564615; DOI=10.1289/ehp.01109801;
RA   Rossi M.R., Masters J.R.W., Park S., Todd J.H., Garrett S.H., Sens M.A.,
RA   Somji S., Nath J., Sens D.A.;
RT   "The immortalized UROtsa cell line as a potential cell culture model
RT   of human urothelium.";
RL   Environ. Health Perspect. 109:801-808(2001).
//
RX   PubMed=19921857; DOI=10.1021/tx900346q;
RA   Somji S., Zhou X.-D., Mehus A., Sens M.A., Garrett S.H., Lutz K.L.,
RA   Dunlevy J.R., Zheng Y., Sens D.A.;
RT   "Variation of keratin 7 expression and other phenotypic
RT   characteristics of independent isolates of cadmium transformed human
RT   urothelial cells (UROtsa).";
RL   Chem. Res. Toxicol. 23:348-356(2010).
//
RX   PubMed=20186695; DOI=10.1002/jat.1513;
RA   Cao L., Zhou X.-D., Sens M.A., Garrett S.H., Zheng Y., Dunlevy J.R.,
RA   Sens D.A., Somji S.;
RT   "Keratin 6 expression correlates to areas of squamous differentiation
RT   in multiple independent isolates of As(+3)-induced bladder cancer.";
RL   J. Appl. Toxicol. 30:416-430(2010).
//
RX   PubMed=23691160; DOI=10.1371/journal.pone.0064139;
RA   Johnen G., Rozynek P., von der Gathen Y., Bryk O., Zdrenka R.,
RA   Johannes C., Weber D.G., Igwilo-Okuefuna O., Raiko I., Hippler J.,
RA   Bruning T., Dopp E.;
RT   "Cross-contamination of a UROtsa stock with T24 cells -- molecular
RT   comparison of different cell lines and stocks.";
RL   PLoS ONE 8:E64139-E64139(2013).
//
RX   PubMed=26783756; DOI=10.1371/journal.pone.0147362;
RA   Slusser-Nore A., Larson-Casey J.L., Zhang R.-W., Zhou X.-D., Somji S.,
RA   Garrett S.H., Sens D.A., Dunlevy J.R.;
RT   "SPARC expression is selectively suppressed in tumor initiating
RT   urospheres isolated from As+3- and Cd+2-transformed human urothelial
RT   cells (UROtsa) stably transfected with SPARC.";
RL   PLoS ONE 11:E0147362-E0147362(2016).
//
RX   PubMed=30550540; DOI=10.1371/journal.pone.0207877;
RA   Hoggarth Z.E., Osowski D.B., Freeberg B.A., Garrett S.H., Sens D.A.,
RA   Sens M.A., Zhou X.-D., Zhang K.K., Somji S.;
RT   "The urothelial cell line UROtsa transformed by arsenite and cadmium
RT   display basal characteristics associated with muscle invasive
RT   urothelial cancers.";
RL   PLoS ONE 13:E0207877-E0207877(2018).
//