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Cellosaurus NCI-H295R (CVCL_0458)

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Cell line name NCI-H295R
Synonyms NCI H295R; NCIH295R; H295R; H-295R; H295R-S1
Accession CVCL_0458
Resource Identification Initiative To cite this cell line use: NCI-H295R (RRID:CVCL_0458)
Comments Characteristics: Grows in a medium called Nu-Serum type I.
Doubling time: 25 hours (PubMed=29371329).
Omics: Genome sequenced.
Omics: SNP array analysis.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Adrenal gland; UBERON=UBERON_0002369.
Sequence variations
  • Mutation; HGNC; 2514; CTNNB1; Simple; p.Ser45Pro (c.133T>C); ClinVar=VCV000017589; Zygosity=Heterozygous (from parent cell line).
Disease Adrenal cortex carcinoma (NCIt: C9325)
Adrenocortical carcinoma (ORDO: Orphanet_1501)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0456 (NCI-H295)
CVCL_5V09 (HAC13)CVCL_S898 (HAC15)CVCL_5V08 (HAC50)
Sex of cell Female
Age at sampling 48Y
Category Cancer cell line
STR profile Source(s): ATCC; CCRID; CLS

Penta D8
Penta E5,12

Run an STR similarity search on this cell line
Web pages https://en.wikipedia.org/wiki/H295R

PubMed=15541570; DOI=10.1016/j.mce.2003.12.020
Rainey W.E., Saner K., Schimmer B.P.
Adrenocortical cell lines.
Mol. Cell. Endocrinol. 228:23-38(2004)

PubMed=18000308; DOI=10.1677/JOE-07-0166
Samandari E., Kempna P., Nuoffer J.-M., Hofer G., Mullis P.-E., Fluck C.E.
Human adrenal corticocarcinoma NCI-H295R cells produce more androgens than NCI-H295A cells and differ in 3beta-hydroxysteroid dehydrogenase type 2 and 17,20 lyase activities.
J. Endocrinol. 195:459-472(2007)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=21924324; DOI=10.1016/j.mce.2011.08.041
Wang T., Rainey W.E.
Human adrenocortical carcinoma cell lines.
Mol. Cell. Endocrinol. 351:58-65(2012)

PubMed=22266826; DOI=10.1055/s-0031-1298019
Wang T., Rowland J.G., Parmar J., Nesterova M., Seki T., Rainey W.E.
Comparison of aldosterone production among human adrenocortical cell lines.
Horm. Metab. Res. 44:245-250(2012)

PubMed=23951072; DOI=10.1371/journal.pone.0071022
Nishi H., Arai H., Momiyama T.
NCI-H295R, a human adrenal cortex-derived cell line, expresses purinergic receptors linked to Ca(2)(+)-mobilization/influx and cortisol secretion.
PLoS ONE 8:E71022-E71022(2013)

PubMed=29371329; DOI=10.1530/ERC-17-0447
Kiseljak-Vassiliades K., Zhang Y., Bagby S.M., Kar A., Pozdeyev N., Xu M., Gowan K., Sharma V., Raeburn C.D., Albuja-Cruz M.B., Jones K.L., Fishbein L., Schweppe R.E., Somerset H., Pitts T.M., Leong S., Wierman M.E.
Development of new preclinical models to advance adrenocortical carcinoma research.
Endocr. Relat. Cancer 25:437-451(2018)

PubMed=33981288; DOI=10.3389/fendo.2021.669426
Rossini E., Tamburello M., Abate A., Beretta S., Fragni M., Cominelli M., Cosentini D., Hantel C., Bono F., Grisanti S., Poliani P.L., Tiberio G.A.M., Memo M., Sigala S., Berruti A.
Cytotoxic effect of progesterone, tamoxifen and their combination in experimental cell models of human adrenocortical cancer.
Front. Endocrinol. 12:669426.1-669426.13(2021)

PubMed=34572026; DOI=10.3390/cells10092376
Wellman K., Fu R., Baldwin A., Rege J., Murphy E., Rainey W.E., Mukherjee N.
Transcriptomic response dynamics of human primary and immortalized adrenocortical cells to steroidogenic stimuli.
Cells 10:2376.1-2376.15(2021)

PubMed=35563746; DOI=10.3390/cells11091439
Sigala S., Bothou C., Penton D., Abate A., Peitzsch M., Cosentini D., Tiberio G.A.M., Bornstein S.R., Berruti A., Hantel C.
A comprehensive investigation of steroidogenic signaling in classical and new experimental cell models of adrenocortical carcinoma.
Cells 11:1439.1-1439.17(2022)

PubMed=35764904; DOI=10.1007/s12020-022-03112-w
Sigala S., Rossini E., Abate A., Tamburello M., Bornstein S.R., Hantel C.
An update on adrenocortical cell lines of human origin.
Endocrine 77:432-437(2022)

Cell line collections (Providers) ATCC; CRL-2128
CLS; 300483
KCB; KCB 2015009YJ
Cell line databases/resources CLO; CLO_0008082
MCCL; MCC:0000355
cancercelllines; CVCL_0458
CCRID; 1101HUM-PUMC000256
Lonza; 378
Anatomy/cell type resources BTO; BTO:0002588
Chemistry resources ChEMBL-Cells; CHEMBL3307998
ChEMBL-Targets; CHEMBL612593
PubChem_Cell_line; CVCL_0458
Encyclopedic resources Wikidata; Q25323725
Experimental variables resources EFO; EFO_0002289
Gene expression databases ArrayExpress; E-MTAB-38
Polymorphism and mutation databases Cosmic; 1172633
Cosmic; 1191070
Cosmic; 1622873
Cosmic; 2364101
Cosmic; 2574494
Progenetix; CVCL_0458
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number27