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Cellosaurus MDBK (CVCL_0421)

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Cell line name MDBK
Synonyms MDBK (NBL-1); NBL-1; Madin-Darby Bovine Kidney; Madin Darby Bovine Kidney
Accession CVCL_0421
Resource Identification Initiative To cite this cell line use: MDBK (RRID:CVCL_0421)
Comments Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Virology: Not susceptible to infection by wild-type and vaccinal strains of myxoma virus (MYXV) (PubMed=17900332).
Doubling time: ~35 hours (DSMZ=ACC-174); ~24 hours (Note=Lot 032699), ~31 hours (Note=Lot 07222019) (JCRB=JCRB9028).
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Species of origin Bos taurus (Bovine) (NCBI Taxonomy: 9913)
Hierarchy Children:
CVCL_DD22 (BHH2C)CVCL_DD23 (BHH3)CVCL_DD24 (BHH8)
CVCL_A9KJ (BTL2013)CVCL_DD19 (CRIB-1)CVCL_DD25 (M2B)
CVCL_E1FE (MDBK CD46 KO)CVCL_DD20 (MDBK clone 955-1)CVCL_DD21 (MDBK clone 958-9)
CVCL_VD89 (MDBK DeltaGPI)CVCL_RT35 (MDBK-221)CVCL_4541 (MDBK-Bu100)
CVCL_A2ZW (MDBK-N4)CVCL_A9LD (MDBK-Nectin-4)CVCL_E073 (MDBK-NST)
CVCL_0I74 (MDBK-PES)CVCL_RX00 (MDBK-t2)CVCL_A2ZX (MDBK-TfR)
CVCL_3021 (MDBK.P3)CVCL_IZ74 (MDBK/441)CVCL_B7IL (MDBK527)
Sex of cell Male
Age at sampling Adult
Category Spontaneously immortalized cell line
Web pages http://www.cells-talk.com/index.php/page/copelibrary?key=MDBK
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/m/cell-lines-detail-133.html
https://www.microscopyu.com/gallery-images/madin-darby-bovine-kidney-epithelial-cells-mdbk-line
Publications

PubMed=13567776; DOI=10.3181/00379727-98-24111
Madin S.H., Darby N.B. Jr.
Established kidney cell lines of normal adult bovine and ovine origin.
Proc. Soc. Exp. Biol. Med. 98:574-576(1958)

PubMed=14207851; DOI=10.1093/jnci/33.2.347
Nelson-Rees W.A., Kniazeff A.J., Darby N.B. Jr.
Preservation of bulk chromatin with decrease in number of chromosomes in cells of an established bovine kidney line.
J. Natl. Cancer Inst. 33:347-361(1964)

PubMed=5988125; DOI=10.1007/BF00326444
Nelson-Rees W.A., Darby N.B. Jr., Siegel S.T.
Clonal derivatives of a bovine kidney cell line (MDBK) and continuing preservation of total chromosomal material.
Chromosoma 18:70-78(1966)

PubMed=5587551; DOI=10.1159/000129961
Nelson-Rees W.A., Kniazeff A.J., Darby N.B. Jr.
Debut and accumulation of centric fusion products: an index to age of certain cell lines.
Cytogenetics 6:436-450(1967)

DOI=10.1007/BF02618370
Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)

PubMed=2580922; DOI=10.1089/jir.1985.5.65
Feinstein S., Traub A., Lazar A., Mizrahi A., Teitz Y.
Studies on cell binding and internalization of human lymphoblastoid (Namalva) interferon.
J. Interferon Res. 5:65-76(1985)

PubMed=17900332; DOI=10.1186/1743-422X-4-94; PMCID=PMC2045666
Pignolet B., Duteyrat J.-L., Allemandou A., Gelfi J., Foucras G., Bertagnoli S.
In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strains.
Virol. J. 4:94.1-94.5(2007)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=24973239; DOI=10.1099/vir.0.065995-0
Richter M., Reimann I., Schirrmeier H., Kirkland P.D., Beer M.
The viral envelope is not sufficient to transfer the unique broad cell tropism of Bungowannah virus to a related pestivirus.
J. Gen. Virol. 95:2216-2222(2014)

PubMed=25565633; DOI=10.1371/journal.pone.0117154; PMCID=PMC4286239
Oelschlegel A.M., Geissen M., Lenk M., Riebe R., Angermann M., Schaetzl H., Groschup M.H.
A bovine cell line that can be infected by natural sheep scrapie prions.
PLoS ONE 10:E0117154-E0117154(2015)

Cross-references
Cell line collections (Providers) ATCC; CCL-22
ATCC; CRL-6071 - Discontinued
BCRC; 60126
BCRJ; 0167
CCLV; CCLV-RIE 0084
CCLV; CCLV-RIE 0261
CCTCC; GDC0108
CCTCC; GDC0290
CLS; 600396
DSMZ; ACC-174
ECACC; 90050801
IZSLER; BS CL 63
JCRB; IFO50014
JCRB; JCRB9028
KCB; KCB 90031YJ
KCLB; 10022
NCBI_Iran; C500
RCB; RCB0180
Cell line databases/resources CLO; CLO_0007642
CLO; CLO_0007643
CLO; CLO_0050865
MCCL; MCC:0000318
CLDB; cl3412
CLDB; cl3413
CLDB; cl3414
CLDB; cl3415
CLDB; cl3416
CCRID; 1101BOV-PUMC000288
CCRID; 1102BOV-NIFDC00076
CCRID; 3101BOVGNO7
CCRID; 5301BOV-KCB90031YJ
DSMZCellDive; ACC-174
FCS-free; 40-4-47-1-8-6
Lonza; 925
Anatomy/cell type resources BTO; BTO_0000836
Encyclopedic resources Wikidata; Q54904675
Entry history
Entry creation04-Apr-2012
Last entry update19-Dec-2024
Version number36