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Cellosaurus Hs 294T (CVCL_0331)

[Text version]
Cell line name Hs 294T
Synonyms HS-294T; Hs-294T; HS-294-T; HS-294; Hs294T; HS294T; Hs294; HS294
Accession CVCL_0331
Resource Identification Initiative To cite this cell line use: Hs 294T (RRID:CVCL_0331)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Population: Caucasian.
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Misspelling: HTB-140; Note=Based on the ATCC catalog number.
Misspelling: HTB140; Note=Based on the ATCC catalog number.
Derived from site: Metastatic; Lymph node; UBERON=UBERON_0000029.
Sequence variations
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (PubMed=15467732; DepMap=ACH-000014).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (PubMed=31068700).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*01:01,25:01
HLA-BB*07:02,08:01
HLA-CC*07:02,07:02
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0.39
East Asian, North0
East Asian, South0
South Asian0.34
European, North72.19
European, South27.07
Disease Melanoma (NCIt: C3224)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_1057 (A101D)CVCL_E3AI (Hs294T R)
Sex of cell Male
Age at sampling 56Y
Category Cancer cell line
STR profile Source(s): ATCC=HTB-140; PubMed=25877200

Markers:
AmelogeninX
CSF1PO11
D2S133817,24
D3S135816,17
D5S81812
D7S82010
D8S117913,14 (ATCC=HTB-140)
14 (PubMed=25877200)
D13S31711,12
D16S53911,12
D18S5113,17
D19S43314,14.2
D21S1130
FGA23,24
Penta D13
Penta E7,10
TH018,9.3
TPOX8,11
vWA16,18

Run an STR similarity search on this cell line
Publications

PubMed=265522; DOI=10.1073/pnas.74.2.565; PMCID=PMC392331
Fabricant R.N., De Larco J.E., Todaro G.J.
Nerve growth factor receptors on human melanoma cells in culture.
Proc. Natl. Acad. Sci. U.S.A. 74:565-569(1977)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=375235; DOI=10.1073/pnas.76.3.1288; PMCID=PMC383236
Sherwin S.A., Sliski A.H., Todaro G.J.
Human melanoma cells have both nerve growth factor and nerve growth factor-specific receptors on their cell surfaces.
Proc. Natl. Acad. Sci. U.S.A. 76:1288-1292(1979)

PubMed=478563; DOI=10.1007/BF02616140
Creasey A.A., Smith H.S., Hackett A.J., Fukuyama K., Epstein W.L., Madin S.H.
Biological properties of human melanoma cells in culture.
In Vitro 15:342-350(1979)

PubMed=6220172
Dracopoli N.C., Fogh J.
Polymorphic enzyme analysis of cultured human tumor cell lines.
J. Natl. Cancer Inst. 70:469-476(1983)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=3335022
Alley M.C., Scudiero D.A., Monks A., Hursey M.L., Czerwinski M.J., Fine D.L., Abbott B.J., Mayo J.G., Shoemaker R.H., Boyd M.R.
Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.
Cancer Res. 48:589-601(1988)

PubMed=8755562; DOI=10.1073/pnas.93.15.7832; PMCID=PMC38834
Liu D.-C., Pearlman E., Diaconu E., Guo K., Mori H., Haqqi T., Markowitz S.D., Willson J.K.V., Sy M.-S.
Expression of hyaluronidase by tumor cells induces angiogenesis in vivo.
Proc. Natl. Acad. Sci. U.S.A. 93:7832-7837(1996)

PubMed=11414198; DOI=10.1007/s004320000207
Lahm H., Andre S., Hoeflich A., Fischer J.R., Sordat B., Kaltner H., Wolf E., Gabius H.-J.
Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.
J. Cancer Res. Clin. Oncol. 127:375-386(2001)

PubMed=15467732; DOI=10.1038/sj.onc.1208152
Tanami H., Imoto I., Hirasawa A., Yuki Y., Sonoda I., Inoue J., Yasui K., Misawa-Furihata A., Kawakami Y., Inazawa J.
Involvement of overexpressed wild-type BRAF in the growth of malignant melanoma cell lines.
Oncogene 23:8796-8804(2004)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. 3rd, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023; PMCID=PMC7339254
Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. 3rd, Kalocsay M., Jane-Valbuena J., Gelfand E.T., Schweppe D.K., Jedrychowski M.P., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

PubMed=39175042; DOI=10.1186/s12964-024-01788-3; PMCID=PMC11342534
Kot M., Simiczyjew A., Wadzynska J., Zietek M., Matkowski R.A., Nowak D.
Characterization of two melanoma cell lines resistant to BRAF/MEK inhibitors (vemurafenib and cobimetinib).
Cell Commun. Signal. 22:410.1-410.21(2024)

Cross-references
Cell line collections (Providers) ATCC; HTB-140
Cell line databases/resources CLO; CLO_0003917
MCCL; MCC:0000227
cancercelllines; CVCL_0331
Cell_Model_Passport; SIDM00800
CGH-DB; 9319-4
DepMap; ACH-000014
Anatomy/cell type resources BTO; BTO:0003467
Biological sample resources BioSample; SAMN03471454
BioSample; SAMN10988350
CRISP screens repositories BioGRID_ORCS_Cell_line; 178
Chemistry resources ChEMBL-Cells; CHEMBL4523539
ChEMBL-Targets; CHEMBL4523570
PharmacoDB; Hs294T_573_2019
PubChem_Cell_line; CVCL_0331
Encyclopedic resources Wikidata; Q54895578
Experimental variables resources EFO; EFO_0006585
Gene expression databases ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
GEO; GSE52246
GEO; GSM206511
GEO; GSM274685
GEO; GSM887096
GEO; GSM888167
Polymorphism and mutation databases Cosmic; 905232
Cosmic; 933000
IARC_TP53; 30047
LiGeA; CCLE_572
Progenetix; CVCL_0331
Sequence databases EGA; EGAS00001000610
Entry history
Entry creation04-Apr-2012
Last entry update10-Sep-2024
Version number44