ID   Chang Liver
AC   CVCL_0238
SY   Chang liver; Chang Liver (HeLa derivative); Chang Cells; Chang; CHL
DR   BTO; BTO:0002049
DR   CLO; CLO_0002414
DR   CLO; CLO_0002415
DR   MCCL; MCC:0000128
DR   CLDB; cl715
DR   CLDB; cl716
DR   CLDB; cl717
DR   CLDB; cl718
DR   BCRC; 60024
DR   BioSample; SAMN03151672
DR   BioSample; SAMN03472417
DR   BioSample; SAMN03472705
DR   CLS; 300139
DR   ECACC; 88021102
DR   JCRB; IFO50016
DR   RCB; RCB0491
DR   TOKU-E; 937
DR   Wikidata; Q54811726
RX   CelloPub=CLPUB00262;
RX   DOI=10.1007/978-1-4757-1647-4_13;
RX   DOI=10.1007/BF02618370;
RX   PubMed=468301;
RX   PubMed=1246601;
RX   PubMed=4864103;
RX   PubMed=5641128;
RX   PubMed=6988327;
RX   PubMed=11416159;
RX   PubMed=13472685;
RX   PubMed=13531635;
RX   PubMed=14185313;
RX   PubMed=18655026;
RX   PubMed=20143388;
RX   PubMed=21656536;
RX   PubMed=23925245;
CC   Problematic cell line: Contaminated. Shown to be a HeLa derivative (PubMed=4864103; PubMed=5641128; PubMed=1246601; PubMed=20143388; PubMed=21656536). Originally thought to originate from normal hepatic cells obtained from a liver biopsy of an adult.
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00002.
CC   Population: African American.
CC   Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
CC   Omics: Genome sequenced (low read coverage).
CC   Omics: Ubiquitination analysis by proteomics.
CC   Discontinued: BCRC; 60024; true.
CC   Discontinued: RCB; RCB0491; true.
CC   Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
ST   Source(s): ATCC; CLS; ECACC; JCRB; PubMed=11416159
ST   Amelogenin: X
ST   CSF1PO: 9,10 (JCRB)
ST   D13S317: 12,13.3 (ATCC; CLS; JCRB)
ST   D13S317: 12,14 (ECACC)
ST   D16S539: 9,10
ST   D18S51: 16
ST   D21S11: 27,28
ST   D3S1358: 15,18
ST   D5S818: 11,12 (JCRB)
ST   D5S818: 12 (ATCC; CLS; ECACC)
ST   D7S820: 8,12
ST   D8S1179: 12,13
ST   FGA: 21
ST   Penta D: 8,15
ST   Penta E: 7,17
ST   TH01: 7
ST   TPOX: 8,12
ST   vWA: 16,17,18 (JCRB)
ST   vWA: 16,18 (ATCC; CLS; ECACC; PubMed=11416159)
DI   NCIt; C27677; Human papillomavirus-related cervical adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0030 ! HeLa
SX   Female
AG   30Y6M
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 05-10-23; Version: 34
RX   CelloPub=CLPUB00262;
RA   Gold M.;
RT   "A conspiracy of cells. One woman's immortal legacy and the medical
RT   scandal it caused.";
RL   (In) ISBN 9780887060991; pp.1-171; State University of New York Press; Albany (1986).
RX   DOI=10.1007/978-1-4757-1647-4_13;
RA   Biedler J.L.;
RT   "Chromosome abnormalities in human tumor cells in culture.";
RL   (In) Human tumor cells in vitro; Fogh J. (eds.); pp.359-394; Springer; New York (1975).
RX   DOI=10.1007/BF02618370;
RA   Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.;
RT   "The animal cell culture collection.";
RL   In Vitro 5:1-16(1970).
RX   PubMed=468301;
RA   Trejdosiewicz L.K., Trejdosiewicz A.J., Ling N.R., Dykes P.W.;
RT   "Growth enhancing property of human monocytes from normal donors and
RT   cancer patients.";
RL   Immunology 37:247-252(1979).
RX   PubMed=1246601; DOI=10.1126/science.1246601;
RA   Nelson-Rees W.A., Flandermeyer R.R.;
RT   "HeLa cultures defined.";
RL   Science 191:96-98(1976).
RX   PubMed=4864103;
RA   Gartler S.M.;
RT   "Genetic markers as tracers in cell culture.";
RL   Natl. Cancer Inst. Monogr. 26:167-195(1967).
RX   PubMed=5641128; DOI=10.1038/217750a0;
RA   Gartler S.M.;
RT   "Apparent Hela cell contamination of human heteroploid cell lines.";
RL   Nature 217:750-751(1968).
RX   PubMed=6988327; DOI=10.1007/BF02831503;
RA   O'Brien S.J., Shannon J.E., Gail M.H.;
RT   "A molecular approach to the identification and individualization of
RT   human and animal cells in culture: isozyme and allozyme genetic
RT   signatures.";
RL   In Vitro 16:119-135(1980).
RX   PubMed=11416159; DOI=10.1073/pnas.121616198;
RA   Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G.,
RA   Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R.,
RA   Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.;
RT   "Short tandem repeat profiling provides an international reference
RT   standard for human cell lines.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001).
RX   PubMed=13472685;
RA   Syverton J.T., McLaren L.C.;
RT   "Human cells in continuous culture. I. Derivation of cell strains from
RT   esophagus, palate, liver, and lung.";
RL   Cancer Res. 17:923-926(1957).
RX   PubMed=13531635; DOI=10.1007/BF00340146;
RA   Pomerat C.M., Kent S.P., Logie L.C.;
RT   "Irradiation of cells in tissue culture. I. Giant cell induction in
RT   strain cultures versus elements from primary explants.";
RL   Z. Zellforsch. Mikrosk. Anat. 47:158-174(1957).
RX   PubMed=14185313; DOI=10.1126/science.146.3641.241;
RG   Cell Culture Collection Committee;
RT   "Animal cell strains. The Cell Culture Collection Committee has
RT   assembled and certified 23 strains of animal cells.";
RL   Science 146:241-243(1964).
RX   PubMed=18655026; DOI=10.1002/pmic.200700887;
RA   Tan F.-W., Lu L.-F., Cai Y., Wang J.-L., Xie Y.-F., Wang L., Gong Y.-H.,
RA   Xu B.-E., Wu J., Luo Y., Qiang B.-Q., Yuan J.-G., Sun X.-Q., Peng X.-Z.;
RT   "Proteomic analysis of ubiquitinated proteins in normal hepatocyte
RT   cell line Chang liver cells.";
RL   Proteomics 8:2885-2896(2008).
RX   PubMed=20143388; DOI=10.1002/ijc.25242;
RA   Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A.,
RA   MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R.,
RA   Freshney R.I.;
RT   "Check your cultures! A list of cross-contaminated or misidentified
RT   cell lines.";
RL   Int. J. Cancer 127:1-8(2010).
RX   PubMed=21656536; DOI=10.1002/hep.24475;
RA   Gao Q., Wang X.-Y., Zhou J., Fan J.;
RT   "Cell line misidentification: the case of the Chang liver cell line.";
RL   Hepatology 54:1894-1895(2011).
RX   PubMed=23925245; DOI=10.1038/nature12064;
RA   Adey A., Burton J.N., Kitzman J.O., Hiatt J.B., Lewis A.P.,
RA   Martin B.K., Qiu R.-L., Lee C., Shendure J.;
RT   "The haplotype-resolved genome and epigenome of the aneuploid HeLa
RT   cancer cell line.";
RL   Nature 500:207-211(2013).