AC   CVCL_0019
SY   SH-Sy5y; SHSY5Y; SHSY-5Y; SK-SH-SY5Y; SY5Y; SH-SY5Y Parental
DR   BTO; BTO:0000793
DR   CLO; CLO_0009015
DR   EFO; EFO_0002717
DR   MCCL; MCC:0000421
DR   CLDB; cl4287
DR   CLDB; cl4288
DR   CLDB; cl4289
DR   CLDB; cl4290
DR   Abcam; ab275475
DR   AddexBio; C0005004/63
DR   ATCC; CRL-2266
DR   BCRJ; 0223
DR   BioGRID_ORCS_Cell_line; 1228
DR   BioSample; SAMN10987717
DR   cancercelllines; CVCL_0019
DR   CCRID; 1101HUM-PUMC000026
DR   CCRID; 4201PAT-CCTCC00086
DR   CCRID; 4201HUM-CCTCC00210
DR   CCRID; 5301HUM-KCB06107YJ
DR   Cell_Model_Passport; SIDM01236
DR   CGH-DB; 63-1
DR   ChEMBL-Cells; CHEMBL3307740
DR   ChEMBL-Targets; CHEMBL614910
DR   CLS; 300154
DR   Cosmic; 688084
DR   Cosmic; 1019933
DR   Cosmic; 1167410
DR   Cosmic; 1212536
DR   Cosmic; 2058109
DR   Cosmic; 2239470
DR   Cosmic; 2393641
DR   DepMap; ACH-001188
DR   DSMZ; ACC-209
DR   DSMZCellDive; ACC-209
DR   ECACC; 94030304
DR   GEO; GSM231608
DR   GEO; GSM231609
DR   GEO; GSM231610
DR   GEO; GSM231611
DR   GEO; GSM231612
DR   GEO; GSM231613
DR   GEO; GSM231614
DR   GEO; GSM231615
DR   GEO; GSM231616
DR   GEO; GSM231617
DR   GEO; GSM231618
DR   GEO; GSM231619
DR   GEO; GSM231620
DR   GEO; GSM231621
DR   GEO; GSM231622
DR   GEO; GSM231623
DR   GEO; GSM231624
DR   GEO; GSM231625
DR   GEO; GSM231626
DR   GEO; GSM231627
DR   GEO; GSM231628
DR   GEO; GSM231629
DR   GEO; GSM231630
DR   GEO; GSM231631
DR   GEO; GSM231632
DR   GEO; GSM231633
DR   GEO; GSM231634
DR   GEO; GSM231635
DR   GEO; GSM231636
DR   GEO; GSM231637
DR   GEO; GSM231638
DR   GEO; GSM231639
DR   GEO; GSM231640
DR   GEO; GSM231641
DR   GEO; GSM231642
DR   GEO; GSM231643
DR   GEO; GSM231644
DR   GEO; GSM231645
DR   GEO; GSM231646
DR   GEO; GSM231647
DR   GEO; GSM231648
DR   GEO; GSM231649
DR   GEO; GSM231650
DR   GEO; GSM231651
DR   GEO; GSM231652
DR   GEO; GSM231653
DR   GEO; GSM231654
DR   GEO; GSM231655
DR   GEO; GSM231656
DR   GEO; GSM231657
DR   GEO; GSM231658
DR   GEO; GSM231659
DR   GEO; GSM231660
DR   GEO; GSM231661
DR   GEO; GSM231662
DR   GEO; GSM231663
DR   GEO; GSM231664
DR   GEO; GSM231665
DR   GEO; GSM231666
DR   GEO; GSM231667
DR   GEO; GSM231668
DR   GEO; GSM231669
DR   GEO; GSM231670
DR   GEO; GSM231671
DR   GEO; GSM231672
DR   GEO; GSM231673
DR   GEO; GSM692874
DR   GEO; GSM887570
DR   GEO; GSM888653
DR   GEO; GSM1622294
DR   GEO; GSM2371253
DR   GEO; GSM2394368
DR   IARC_TP53; 23599
DR   ICLC; HTL95013
DR   KCB; KCB 2006107YJ
DR   KCLB; 22266
DR   Kerafast; ECP004
DR   Lonza; 132
DR   MetaboLights; MTBLS455
DR   NCBI_Iran; C611
DR   PharmacoDB; SHSY5Y_1374_2019
DR   PRIDE; PXD003105
DR   PRIDE; PXD003914
DR   PRIDE; PXD004452
DR   PRIDE; PXD010005
DR   PRIDE; PXD010027
DR   PRIDE; PXD010776
DR   PRIDE; PXD014381
DR   PRIDE; PXD020389
DR   PRIDE; PXD029012
DR   Progenetix; CVCL_0019
DR   PubChem_Cell_line; CVCL_0019
DR   TOKU-E; 3118
DR   Ubigene; YC-D014
DR   Wikidata; Q7390126
RX   PubMed=1354203;
RX   PubMed=2535691;
RX   PubMed=2846152;
RX   PubMed=3968181;
RX   PubMed=4748425;
RX   PubMed=6137586;
RX   PubMed=7687927;
RX   PubMed=10630978;
RX   PubMed=11668190;
RX   PubMed=12505268;
RX   PubMed=14676279;
RX   PubMed=15150091;
RX   PubMed=15720811;
RX   PubMed=16120276;
RX   PubMed=16822308;
RX   PubMed=17506115;
RX   PubMed=18957096;
RX   PubMed=20655465;
RX   PubMed=22213050;
RX   PubMed=22460905;
RX   PubMed=23421552;
RX   PubMed=24466371;
RX   PubMed=25182563;
RX   PubMed=25730103;
RX   PubMed=25884760;
RX   PubMed=26342562;
RX   PubMed=26972028;
RX   PubMed=27141528;
RX   PubMed=28118852;
RX   PubMed=28350380;
RX   PubMed=28601559;
RX   PubMed=29468137;
RX   PubMed=30894373;
RX   PubMed=31068700;
CC   Problematic cell line: Partially contaminated. Some laboratories that are redistributing this cell line are in fact redistributing a contaminated cell line of mouse origin (PubMed=25182563).
CC   Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
CC   Part of: ENCODE project common cell types; tier 3.
CC   From: Memorial Sloan Kettering Cancer Center; New York; USA.
CC   Registration: Memorial Sloan Kettering Cancer Center Office of Technology Development; SK 810.
CC   Population: Caucasian.
CC   Characteristics: Neuroblastic type (N-type) (PubMed=15720811).
CC   Characteristics: There seem to be differences in the retinoic acid (RA)-induced neuronal phenotype of SH-SY5Y cells from ATCC and ECACC. After 5 days of RA treatment, ECACC cells are slightly larger in size and contains significant amount of neuroblastic (N-type) cells and a small fraction of epithelial (S-type) cells (PubMed=18957096).
CC   Virology: Low susceptibility to infection by Zika virus (ZIKV) (PubMed=29468137).
CC   Doubling time: >55 hours (DSMZ=ACC-209).
CC   Sequence variation: Mutation; HGNC; 427; ALK; Simple; p.Phe1174Leu (c.3522C>A); ClinVar=VCV000217852; Zygosity=Unspecified (PubMed=28350380).
CC   Omics: Deep antibody staining analysis.
CC   Omics: Deep exome analysis.
CC   Omics: Deep proteome analysis.
CC   Omics: Deep tyrosine phosphoproteome analysis.
CC   Omics: GPI-anchored proteins analysis by proteomics.
CC   Omics: Mitochondrial proteome analysis by 2D-DE/MS.
CC   Omics: N-glycan profiling.
CC   Omics: SNP array analysis.
CC   Omics: Transcriptome analysis by microarray.
CC   Omics: Transcriptome analysis by RNAseq.
CC   Genome ancestry: African=0%; Native American=1.17%; East Asian, North=0.35%; East Asian, South=0.1%; South Asian=0.08%; European, North=69.09%; European, South=29.2% (PubMed=30894373).
CC   Misspelling: SH-SY5; Note=Occasionally.
CC   Derived from site: Metastatic; Bone marrow; UBERON=UBERON_0002371.
ST   Source(s): ATCC; CCRID; CLS; DSMZ; ECACC; Genomics_Center_BCF_Technion; KCLB
ST   Amelogenin: X
ST   CSF1PO: 11
ST   D10S1248: 14
ST   D12S391: 18,22
ST   D13S317: 11
ST   D16S539: 8,13
ST   D18S51: 13,16
ST   D19S433: 13,14
ST   D1S1656: 12
ST   D21S11: 31,31.2
ST   D22S1045: 16,17
ST   D2S1338: 17,19
ST   D2S441: 11,11.3
ST   D3S1358: 15,16
ST   D5S818: 12
ST   D6S1043: 12,18
ST   D7S820: 7,10
ST   D8S1179: 15
ST   FGA: 23.2,24
ST   Penta D: 10,12
ST   Penta E: 7,11
ST   TH01: 7,10
ST   TPOX: 8,11
ST   vWA: 14,18
DI   NCIt; C3270; Neuroblastoma
DI   ORDO; Orphanet_635; Neuroblastoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0531 ! SK-N-SH
SX   Female
AG   4Y
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 30-01-24; Version: 44
RX   PubMed=1354203; DOI=10.1002/ijc.2910520116;
RA   Gross N., Favre S., Beck D., Meyer M.;
RT   "Differentiation-related expression of adhesion molecules and
RT   receptors on human neuroblastoma tissues, cell lines and variants.";
RL   Int. J. Cancer 52:85-91(1992).
RX   PubMed=2535691;
RA   Ciccarone V., Spengler B.A., Meyers M.B., Biedler J.L., Ross R.A.;
RT   "Phenotypic diversification in human neuroblastoma cells: expression
RT   of distinct neural crest lineages.";
RL   Cancer Res. 49:219-225(1989).
RX   PubMed=2846152;
RA   Preis P.N., Saya H., Nadasdi L., Hochhaus G., Levin V., Sadee W.;
RT   "Neuronal cell differentiation of human neuroblastoma cells by
RT   retinoic acid plus herbimycin A.";
RL   Cancer Res. 48:6530-6534(1988).
RX   PubMed=3968181; DOI=10.1083/jcb.100.2.588;
RA   Meyers M.B., Spengler B.A., Chang T.-D., Melera P.W., Biedler J.L.;
RT   "Gene amplification-associated cytogenetic aberrations and protein
RT   changes in vincristine-resistant Chinese hamster, mouse, and human
RT   cells.";
RL   J. Cell Biol. 100:588-597(1985).
RX   PubMed=4748425;
RA   Biedler J.L., Helson L., Spengler B.A.;
RT   "Morphology and growth, tumorigenicity, and cytogenetics of human
RT   neuroblastoma cells in continuous culture.";
RL   Cancer Res. 33:2643-2652(1973).
RX   PubMed=6137586; DOI=10.1093/jnci/71.4.741;
RA   Ross R.A., Spengler B.A., Biedler J.L.;
RT   "Coordinate morphological and biochemical interconversion of human
RT   neuroblastoma cells.";
RL   J. Natl. Cancer Inst. 71:741-747(1983).
RX   PubMed=7687927;
RA   Xu Y.-Y., Wands J.R., de la Monte S.M.;
RT   "Characterization of thread proteins expressed in neuroectodermal
RT   tumors.";
RL   Cancer Res. 53:3823-3829(1993).
RX   PubMed=10630978; DOI=10.1667/0033-7587(2000)153[0062:FORIMI]2.0.CO;2;
RA   Akudugu J.M., Slabbert J.P., Serafin A., Bohm L.;
RT   "Frequency of radiation-induced micronuclei in neuronal cells does not
RT   correlate with clonogenic survival.";
RL   Radiat. Res. 153:62-67(2000).
RX   PubMed=11668190; DOI=10.1177/002215540104901105;
RA   Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.;
RT   "Immunocytochemical analysis of cell lines derived from solid
RT   tumors.";
RL   J. Histochem. Cytochem. 49:1369-1378(2001).
RX   PubMed=12505268; DOI=10.1016/s0165-4608(02)00523-x;
RA   Spengler B.A., Biedler J.L., Ross R.A.;
RT   "A corrected karyotype for the SH-SY5Y human neuroblastoma cell
RT   line.";
RL   Cancer Genet. Cytogenet. 138:177-178(2002).
RX   PubMed=14676279; DOI=10.1242/jcs.00834;
RA   Goldschneider D., Blanc E., Raguenez G., Barrois M., Legrand A.,
RA   Le Roux G., Haddada H., Benard J., Douc-Rasy S.;
RT   "Differential response of p53 target genes to p73 overexpression in
RT   SH-SY5Y neuroblastoma cell line.";
RL   J. Cell Sci. 117:293-301(2004).
RX   PubMed=15150091; DOI=10.1158/0008-5472.CAN-03-0809;
RA   Schaefer K.-L., Brachwitz K., Wai D.H., Braun Y., Diallo R.,
RA   Korsching E., Eisenacher M., Voss R., van Valen F., Baer C., Selle B.,
RA   Spahn L., Liao S.-K., Lee K.A.W., Hogendoorn P.C.W., Reifenberger G.,
RA   Gabbert H.E., Poremba C.;
RT   "Expression profiling of t(12;22) positive clear cell sarcoma of soft
RT   tissue cell lines reveals characteristic up-regulation of potential
RT   new marker genes including ERBB3.";
RL   Cancer Res. 64:3395-3405(2004).
RX   PubMed=15720811; DOI=10.1593/neo.04310;
RA   Walton J.D., Kattan D.R., Thomas S.K., Spengler B.A., Guo H.-F.,
RA   Biedler J.L., Cheung N.-K.V., Ross R.A.;
RT   "Characteristics of stem cells from human neuroblastoma cell lines and
RT   in tumors.";
RL   Neoplasia 6:838-845(2004).
RX   PubMed=16120276; DOI=10.1016/S1567-7249(01)00007-1;
RA   Scheffler N.K., Miller S.W., Carroll A.K., Anderson C., Davis R.E.,
RA   Ghosh S.S., Gibson B.W.;
RT   "Two-dimensional electrophoresis and mass spectrometric identification
RT   of mitochondrial proteins from an SH-SY5Y neuroblastoma cell line.";
RL   Mitochondrion 1:161-179(2001).
RX   PubMed=16822308; DOI=10.1186/1471-2407-6-177;
RA   Dam V., Morgan B.T., Mazanek P., Hogarty M.D.;
RT   "Mutations in PIK3CA are infrequent in neuroblastoma.";
RL   BMC Cancer 6:177.1-177.10(2006).
RX   PubMed=17506115; DOI=10.1002/nbm.1181;
RA   Peet A.C., McConville C.M., Wilson M.P., Levine B.A., Reed M., Dyer S.A.,
RA   Edwards E.C., Strachan M.C., McMullan D.J., Wilkes T.M., Grundy R.G.;
RT   "1H MRS identifies specific metabolite profiles associated with
RT   MYCN-amplified and non-amplified tumour subtypes of neuroblastoma cell
RT   lines.";
RL   NMR Biomed. 20:692-700(2007).
RX   PubMed=18957096; DOI=10.1186/1756-0500-1-95;
RA   Nishida Y., Adati N., Ozawa R., Maeda A., Sakaki Y., Takeda T.;
RT   "Identification and classification of genes regulated by
RT   phosphatidylinositol 3-kinase- and TRKB-mediated signalling pathways
RT   during neuronal differentiation in two subtypes of the human
RT   neuroblastoma cell line SH-SY5Y.";
RL   BMC Res. Notes 1:95.1-95.11(2008).
RX   PubMed=20655465; DOI=10.1016/j.cell.2010.06.004;
RA   Holzel M., Huang S.-D., Koster J., Ora I., Lakeman A., Caron H.N.,
RA   Nijkamp W., Xie J., Callens T., Asgharzadeh S., Seeger R.C.,
RA   Messiaen L.M., Versteeg R., Bernards R.;
RT   "NF1 is a tumor suppressor in neuroblastoma that determines retinoic
RT   acid response and disease outcome.";
RL   Cell 142:218-229(2010).
RX   PubMed=22213050; DOI=10.1002/ijc.27415;
RA   Gawecka J.E., Geerts D., Koster J., Caliva M.J., Sulzmaier F.J.,
RA   Opoku-Ansah J., Wada R.K., Bachmann A.S., Ramos J.W.;
RT   "PEA15 impairs cell migration and correlates with clinical features
RT   predicting good prognosis in neuroblastoma.";
RL   Int. J. Cancer 131:1556-1568(2012).
RX   PubMed=22460905; DOI=10.1038/nature11003;
RA   Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A.,
RA   Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A.,
RA   Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J.,
RA   Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E.,
RA   Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J.,
RA   Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C.,
RA   Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C.,
RA   Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P.,
RA   Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L.,
RA   Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R.,
RA   Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.;
RT   "The Cancer Cell Line Encyclopedia enables predictive modelling of
RT   anticancer drug sensitivity.";
RL   Nature 483:603-607(2012).
RX   PubMed=23421552; DOI=10.1089/ast.2012.0822;
RA   Zhang Y.-Q., Wang H.-B., Lai C.-J., Wang L., Deng Y.-L.;
RT   "Comparative proteomic analysis of human SH-SY5Y neuroblastoma cells
RT   under simulated microgravity.";
RL   Astrobiology 13:143-150(2013).
RX   PubMed=24466371; DOI=10.1593/tlo.13544;
RA   Loschmann N., Michaelis M., Rothweiler F., Zehner R., Cinatl J.,
RA   Voges Y., Sharifi M., Riecken K., Meyer J., von Deimling A.,
RA   Fichtner I., Ghafourian T., Westermann F., Cinatl J. Jr.;
RT   "Testing of SNS-032 in a panel of human neuroblastoma cell lines with
RT   acquired resistance to a broad range of drugs.";
RL   Transl. Oncol. 6:685-696(2013).
RX   PubMed=25182563; DOI=10.1007/s13577-014-0096-9;
RA   Jiang T., Wang H.-L.;
RT   "Identification of cross-contamination in SH-SY5Y cell line.";
RL   Hum. Cell 27:176-178(2014).
RX   PubMed=25730103; DOI=10.1021/pr5011718;
RA   Hu Y.-L., Mayampurath A., Khan S., Cohen J.K., Mechref Y.,
RA   Volchenboum S.L.;
RT   "N-linked glycan profiling in neuroblastoma cell lines.";
RL   J. Proteome Res. 14:2074-2081(2015).
RX   PubMed=25884760; DOI=10.1371/journal.pcbi.1004130;
RA   Palacios-Moreno J., Foltz L., Guo A.-L., Stokes M.P., Kuehn E.D.,
RA   George L., Comb M.J., Grimes M.L.;
RT   "Neuroblastoma tyrosine kinase signaling networks involve FYN and LYN
RT   in endosomes and lipid rafts.";
RL   PLoS Comput. Biol. 11:e1004130.1-e1004130.33(2015).
RX   PubMed=26342562; DOI=10.1016/j.scr.2015.08.008;
RA   Ross R.A., Walton J.D., Han D., Guo H.-F., Cheung N.-K.V.;
RT   "A distinct gene expression signature characterizes human
RT   neuroblastoma cancer stem cells.";
RL   Stem Cell Res. 15:419-426(2015).
RX   PubMed=26972028; DOI=10.1016/j.jprot.2016.03.008;
RA   Masuishi Y., Kimura Y., Arakawa N., Hirano H.;
RT   "Identification of glycosylphosphatidylinositol-anchored proteins and
RT   omega-sites using TiO2-based affinity purification followed by
RT   hydrogen fluoride treatment.";
RL   J. Proteomics 139:77-83(2016).
RX   PubMed=27141528; DOI=10.1016/j.dib.2016.04.001;
RA   Masuishi Y., Kimura Y., Arakawa N., Hirano H.;
RT   "Data for identification of GPI-anchored peptides and omega-sites in
RT   cancer cell lines.";
RL   Data Brief 7:1302-1305(2016).
RX   PubMed=28118852; DOI=10.1186/s13024-017-0149-0;
RA   Xicoy H., Wieringa B., Martens G.J.M.;
RT   "The SH-SY5Y cell line in Parkinson's disease research: a systematic
RT   review.";
RL   Mol. Neurodegener. 12:10.1-10.11(2017).
RX   PubMed=28350380; DOI=10.1038/sdata.2017.33;
RA   Harenza J.L., Diamond M.A., Adams R.N., Song M.M., Davidson H.L.,
RA   Hart L.S., Dent M.H., Fortina P., Reynolds C.P., Maris J.M.;
RT   "Transcriptomic profiling of 39 commonly-used neuroblastoma cell
RT   lines.";
RL   Sci. Data 4:170033-170033(2017).
RX   PubMed=28601559; DOI=10.1016/j.cels.2017.05.009;
RA   Bekker-Jensen D.B., Kelstrup C.D., Batth T.S., Larsen S.C.,
RA   Haldrup C., Bramsen J.B., Sorensen K.D., Hoyer S., Orntoft T.F.,
RA   Andersen C.L., Nielsen M.L., Olsen J.V.;
RT   "An optimized shotgun strategy for the rapid generation of
RT   comprehensive human proteomes.";
RL   Cell Syst. 4:587-599.e4(2017).
RX   PubMed=29468137; DOI=10.5501/wjv.v7.i1.10;
RA   Himmelsbach K., Hildt E.;
RT   "Identification of various cell culture models for the study of Zika
RT   virus.";
RL   World J. Virol. 7:10-20(2018).
RX   PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747;
RA   Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.;
RT   "An interactive resource to probe genetic diversity and estimated
RT   ancestry in cancer cell lines.";
RL   Cancer Res. 79:1263-1273(2019).
RX   PubMed=31068700; DOI=10.1038/s41586-019-1186-3;
RA   Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C.,
RA   McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X.,
RA   Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F.,
RA   Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R.,
RA   Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C.,
RA   Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A.,
RA   Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D.,
RA   Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L.,
RA   Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A.,
RA   Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D.,
RA   Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M.,
RA   Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R.,
RA   Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A.,
RA   Sellers W.R.;
RT   "Next-generation characterization of the Cancer Cell Line
RT   Encyclopedia.";
RL   Nature 569:503-508(2019).